甲状旁腺激素预防双膦酸盐性颌骨坏死的动物实验研究  

Animal experimentation on preventing bisphosphonate-related osteonecrosis of the jaw with parathyroid hormone

作　　者：万媛 孙翼 蒋宝鑫 张诺 WAN Yuan;SUN Yi;JIANG Baoxin;ZHANG Nuo(School of Stomatology,Wannan Medical College,Wuhu 241002,Anhui,China)

机构地区：[1]皖南医学院口腔医学院,安徽芜湖241002

出　　处：《皖南医学院学报》2024年第3期271-274,共4页Journal of Wannan Medical College

基　　金：皖南医学院中青年科研基金项目(WK202007)。

摘　　要：目的:通过建立小鼠模型探究甲状旁腺激素(PTH)对双膦酸盐性颌骨坏死(BRONJ)发生、发展的影响。方法:采用长期腹腔注射唑来膦酸(ZOL)与地塞米松(DXMS)建立高危SD大鼠模型,采用有创拔牙的方式刺激颌骨,以有效地引发BRONJ样疾病的发展。拔牙后治疗组预防性皮下注射PTH,实验组及空白组不作处理。结果:在拔牙创面的刺激下,成功采用ZOL和DEX联合用药建立BRONJ模型。实验组骨小梁厚度、密度以及数量均较空白组降低(P<0.05),而骨小梁分离度上升(P<0.05);治疗组骨小梁厚度、密度以及数量均较实验组上升(P<0.05),而骨小梁分离度下降(P<0.05)。结论:本研究成功建立了大鼠BRONJ模型,证实PTH可在一定程度上抑制BRONJ的发生,对BRONJ的治疗有一定的效果。Objective:To investigate the effect of parathyroid hormone(PTH)on the development of bisphosphonate-related osteonecrosis of the jaw(BRONJ)in rat models.Methods:BRONJ-like disease was induced by tooth extraction stimulation in SD rats with long-term intraperitoneal injection of zoledronate(ZOL)and dexamethasone(DXMS).Then PTH was subcutaneously and prophylactically applied to the rats in treatment group,whereas rats in the experimental group and control group were in blank intervention.Results:BRONJ model was successfully established by combining zoledronate and dexamethasone under the stimulation of tooth extraction wound.The thickness,density and the number of trabeculae were decreased in rats in the experimental group,yet trabecular separation was increased compared with the blank group(all P<0.05).The thickness,density and the number of trabeculae were increased in rats in the treatment group,whereas trabecular separation was decreased compared to the experimental group(all P<0.05).Conclusion:The BRONJ rat model was successfully established,and parathyroid hormone was confirmed to inhibit the occurrence of BRONJ to a certain extent,and produce certain effect on the treatment of BRONJ.

关 键 词：双膦酸盐性颌骨坏死 动物模型 甲状旁腺激素 

分 类 号：R-332[医药卫生] R581R782