乙型肝炎病毒相关性肝细胞肝癌患者血清miR-125a-5p、miR-127-3p表达及临床意义  

Serum miR-125a-5p,miR-127-3p expression and clinical significance in patients with hepatitis B virus-associated hepatocellular carcinoma

作　　者：王继绪 张国栋 陈茂丽 韩永峰 WANG Jixu;ZHANG Guodong;CHEN Maoli;HAN Yongfeng(Department of Infectious Diseases,Shandong First Medical University Affiliated People′s Hospital,Ji′nan,Shangdong 271199,China)

机构地区：[1]山东第一医科大学附属人民医院感染科,山东济南271199

出　　处：《国际检验医学杂志》2024年第15期1860-1866,共7页International Journal of Laboratory Medicine

基　　金：山东省医药卫生科技发展计划(2020WS063)。

摘　　要：目的探讨乙型肝炎病毒(HBV)相关性肝细胞肝癌(HCC)患者血清微小核糖核酸(miR)-125a-5p、miR-127-3p表达及临床意义。方法选取2018年1月至2020年10月该院收治的90例HBV相关性HCC患者作为HBV相关性HCC组。另选取同期该院的90例体检健康者作为对照组。采用实时荧光定量聚合酶链反应检测血清miR-125a-5p、miR-127-3p表达。分析miR-125a-5p、miR-127-3p表达与HBV相关性HCC患者病理特征的关系。根据HBV相关性HCC患者血清miR-125a-5p、miR-127-3p表达的平均值分为高、低表达组,通过Kaplan-Meier法绘制各组不同的生存曲线。通过Cox回归分析影响HBV相关性HCC患者预后的因素,受试者工作特征(ROC)曲线分析血清miR-125a-5p、miR-127-3p表达对HBV相关性HCC患者死亡的预测价值。结果HBV相关性HCC组血清miR-125a-5p、miR-127-3p相对表达水平低于对照组,差异有统计学意义(P<0.05)。不同肿瘤最大径、分化程度、脉管侵犯、TNM分期、淋巴结转移的HBV相关性HCC患者血清miR-125a-5p、miR-127-3p表达比较差异有统计学意义(P<0.05)。Kaplan-Meier生存曲线分析显示,miR-125a-5p高表达组3年总生存率(64.58%)高于miR-125a-5p低表达组(38.10%),miR-127-3p高表达组3年总生存率(66.00%)高于miR-127-3p低表达组(35.00%),差异有统计学意义(χ^(2)=7.770、9.507,P=0.005、0.002)。HBV相关性HCC患者死亡的独立危险因素为肿瘤最大径≥5 cm、低分化、有脉管侵犯、TNM分期Ⅲ~Ⅳ期、有淋巴结转移,其独立保护因素为miR-125a-5p、miR-127-3p升高。ROC曲线分析结果显示,血清miR-125a-5p、miR-127-3p表达联合预测的曲线下面积(AUC)为0.907,高于血清miR-125a-5p、miR-127-3p表达单独预测的AUC(0.790、0.787),差异有统计学意义(Z=2.691、3.152,P=0.007、0.002)。结论HBV相关性HCC患者血清miR-125a-5p、miR-127-3p低表达,与肿瘤最大径、分化程度、脉管侵犯、TNM分期和预后有关,血清miR-125a-5p、miR-127-3p表达联合对HBV相关�Objective To investigate the serum microRNA(miR)-125a-5p,miR-127-3p expression and clinical significance in patients with hepatitis B virus(HBV)-associated hepatocellular carcinoma(HCC).Methods A total of 90 HBV-associated HCC patients admitted to a hospital from January 2018 to October 2020 were selected as the HBV-associated HCC group.Another 90 healthy subjects from the same period were selected as the control group.Serum miR-125a-5p and miR-127-3p expressions were detected by real-time fluorescence quantitative polymerase chain reaction.To analyze the relationship between miR-125a-5p and miR-127-3p expression and pathological features of patients with HBV-associated HCC.Patients with HBV-associated HCC were divided into high and low expression groups according to the mean values of serum miR-125a-5p and miR-127-3p expression,and the different survival curves of each groups were plotted by the Kaplan-Meier method.Factors affecting the prognosis of patients with HBV-associated HCC were analyzed by Cox regression,and the predictive value of serum miR-125a-5p and miR-127-3p expression on the death of patients with HBV-associated HCC was analyzed by receiver operating characteristic(ROC)curve.Results The relative expression levels of miR-125a-5p and miR-127-3p in HBV-associated HCC group were lower than those in control group,with statistical significance(P<0.05).There were significant differences in the expression of miR-125a-5p and miR-127-3p in serum of HBV-associated HCC patients with different tumor size,differentiation degree,vascular invasion,TNM stage and lymph node metastasis(P<0.05).Kaplan-Meier survival curve analysis showed that the 3-year overall survival rate(64.58%)of the group with high expression of miR-125a-5p was higher than that of the group with low expression of miR-125a-5p(38.10%).The 3-year overall survival rate of miR-127-3p high expression group(66.00%)was higher than that of miR-127-3p low expression group(35.00%),and the difference was statistically significant(χ^(2)=7.770,9.507,P=0.00

关 键 词：乙型肝炎病毒相关性肝细胞肝癌 微小核糖核酸-125a-5p 微小核糖核酸-127-3p 病理特征 预后 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]