1株野生高效纤维素降解菌筛选、鉴定其酶活力测定  

Screening and Identification of a Wild Cellulose-degrading Strain with High Efficiency and Determination of Its Enzyme Activity

作　　者：廖凯 肖畑 颜健红 郑慧新 敬媛蓉[1] 刘秀[1] 倪弦之 金晨钟[1] 郭开发 LIAO Kai;XIAO Tian;YAN Jian-hong;ZHENG Hui-xin;JING Yuan-rong;LIU Xiu;NI Xian-zhi;JIN Chen-zhong;GUO Kai-fa(Key Laboratory of Green Prevention and Control for Pests,Hunan University of Humanities and Technology,Loudi,Hunan 417000,China;Loudi Bureau of Agriculture and Rural Affairs,Loudi,Hunan 417000,China)

机构地区：[1]湖南人文科技学院/有害生物绿色防控重点实验室,湖南娄底417000 [2]娄底市农业农村局,湖南娄底417000

出　　处：《福建农业科技》2024年第5期16-19,共4页Fujian Agricultural Science and Technology

基　　金：农药无害化应用湖南省普通高等学校科技创新团队(2024年);湖南省区域联合基金项目(2023JJ50466、2024JJ7279);湖南省教育厅重点项目(22A0609);娄底市2023年度科技创新研发项目(2024年);湖南省研究生创新项目(CX20231271);湖南省大学生创新创业训练项目(S202310553003X)。

摘　　要：为从腐熟的土壤中分离出产纤维素酶的细菌,并进一步研究该菌株的纤维素降解能力。采用羧甲基纤维素(CMC)-刚果红染色法,筛选出1株野生纤维素降解菌ZG2-3,采用16S rRNA分子鉴定其种类,同时从促腐剂中筛选出4株商品纤维素降解菌,比较野生纤维素降解菌与商品纤维素降解菌的酶活差异。结果表明:从娄底市珠山公园桂花树林下腐熟的土壤中采集分离,通过羧甲基纤维素(CMC)-刚果红染色法,筛选得到了纤维素降解能力较高的1株细菌ZG2-3。经鉴定ZG2-3为枯草芽孢杆菌Bacillus subtilis。ZG2-3的羧甲基纤维素酶(CMCase)活力和滤纸酶(FPA)活力分别为0.054 IU·mL^(-1)和0.02 IU·mL^(-1),其中ZG2-3的CMCase活力显著高于商品纤维素降解菌。In order to isolate cellulase-producing bacteria from the overrotten soil and further study the cellulose degradation ability of the strain,a wild cellulose-degrading strain ZG2-3 was screened by using the carboxymethyl cellulose(CMC)-congo red staining method.Then,the 16 S rRNA molecular identification was used to identify the species,and four commercial cellulose-degrading bacteria strains were screened from the promoting-decomposing agent.Last,the enzyme activity differences between the wild cellulose-degrading bacteria and the commercial cellulose-degrading bacteria were compared.The results showed that one strain of bacteria with high cellulose degradation ability,ZG2-3,was screened by using the carboxymethyl cellulose(CMC)-congo red staining method,which was collected and isolated from the overrotten soil under the osmanthus forest in Zhushan Park,Loudi City.ZG2-3 was identified as Bacillus subtilis.The carboxymethyl cellulase(CMCase)activity and filter paper enzyme(FPA)activity of ZG2-3 were 0.054 IU·mL^(-1)and 0.02 IU·mL^(-1),respectively,and the CMCase activity of ZG2-3 was significantly higher than that of the commercial cellulose-degrading bacteria.

关 键 词：纤维素 枯草芽孢杆菌 筛选 鉴定 酶活力 

分 类 号：S141.4[农业科学—肥料学]