不同性别东乡绿壳蛋鸡血液全基因组甲基化差异分析  

Analysis of genome-wide methylation variance in blood of Dongxiang blue-eggshell chickens with different sexes

作　　者：易兰兰 徐维维 李智鹏 许宏[2] 杨明华[1] 黄英[1] 潘洪彬[1] 李卫真 赵素梅[1] YI Lanlan;XU Weiwei;LI Zhipeng;XU Hong;YANG Minghua;HUANG Ying;PAN Hongbin;LI Weizhen;ZHAO Sumei(College of Animal Science and Technology,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China;School of Economics,Yunnan University of Finance and Economics,Kunming,Yunnan Province 650221,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming,Yunnan Province 650201,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南财经大学经济学院,云南昆明650221 [3]云南农业大学动物医学院,云南昆明650201

出　　处：《中国饲料》2024年第15期49-56,共8页China Feed

基　　金：州校合作项目(2021533416000035);云南省科技重点项目(202202AE090032);国家自然科学基金项目(31760645,31260592,31060331)。

摘　　要：通过对不同性别东乡绿壳蛋鸡的全基因组甲基化的差异分析,检测差异甲基化区域(DMR),分析KEGG通路,为东乡绿壳蛋鸡表观遗传学研究提供参考。试验选取6只120日龄的东乡绿壳蛋鸡,分为公鸡组(LE_COCK)和母鸡组(LE_HEN),每组3只鸡,采集血液提取DNA进行全基因组亚硫酸氢盐测序(WGBS)分析。结果表明:东乡绿壳蛋鸡全基因组上的mC率母鸡和公鸡分别为2.27%和2.03%,母鸡比公鸡高0.24个百分点(P>0.05);CG岛是发生甲基化的主要序列类型,母鸡比公鸡高4.33个百分点(P>0.05);CHG和CHH甲基化类型低于0.11%;CHG甲基化位点的第2位偏向于A;CHH甲基化位点的第2位偏向于A,第3位偏向于T;在CG类型序列中,内含子和3’UTR区域的甲基化水平均高于15%;5’UTR和外显子区域甲基化水平不足10%;16,25号常染色体和一条性染色体甲基化水平较高。东乡绿壳蛋母鸡甲基化水平更高的DMR相关基因在黏附斑,ECM受体互作通路,肌动蛋白细胞骨架的调控,MAPK信号通路等信号通路富集。东乡绿壳蛋公鸡甲基化水平更高的DMR相关基因主要在类固醇生物合成,Notch信号通路,泛素介导的蛋白降解等信号通路富集。研究表明不同性别东乡绿壳蛋鸡具有不同的DMR甲基化特征,母鸡骨骼肌生长发育通路DMR相关基因甲基化水平高于公鸡,而卵泡形成和雌激素相关通路的DMR相关基因甲基化水平低于公鸡。The purpose of this study was to identify differentially methylated regions(DMRs)and analyze KEGG pathways through the genome-wide methylation differential analysis of Dongxiang blue-eggshell chickens of different sexes,and provide a reference for the epigenetics study of these chickens.Six 120 d Dongxiang blue-eggshell chickens were selected for the experiment and divided into cock group(LE_COCK)and hen group(LE_HEN),with 3 chickens in each group.Blood was collected to extract DNA for whole-genome bisulfite sequencing(WGBS)analysis.The results showed that the mC rate in the whole genome of Dongxiang blue-eggshell chickens was 2.27% and 2.03%in hens and cocks,respectively,and hens were 0.24 percentage points higher than cocks(P>0.05).CG islands were the main sequence types of methylation,and hens were 4.33 percentage points higher than cocks(P>0.05).CHG and CHH methylation types were less than 0.11%;the second position of CHG methylation site is preferentially a A;the second position of CHH methylation site was preferentially a A,and the third position was preferentially a T;in the CG type sequence,the methylation level of intron and 3'UTR region was higher than 15%;the methylation level of 5'UTR and exon region was less than 10%;autosomes 16,25 and one sex chromosome were highly methylated.DMR-related genes with higher methylation levels in female Dongxiang blue-eggshell chickens were mainly enriched in focal adhesion,extracellular matrix(ECM)-receptor interaction,regulation of actin cytoskeleton,and mitogen-activated protein kinase(MAPK)signaling pathway.DMR-related genes with higher methylation levels in male Dongxiang blue-eggshell chickens were mainly enriched in steroid biosynthesis,Notch signaling pathway,ubiquitin mediated proteolysis.Studies have shown that different sexes of Dongxiang blue-eggshell chicken have different DMR methylation characteristics.The methylation levels of DMR-related genes in skeletal muscle growth and development pathways in hens were higher than those in cocks,while the methylat

关 键 词：东乡绿壳蛋鸡 血液 WGBS技术 DNA甲基化 

分 类 号：S813.1[农业科学—畜牧学]