山花黄芩提取物对LPS刺激肉鸡肠道形态、抗氧化及免疫功能的影响  

Effects of chlorogenic acid and baicalin on intestinal morphology,antioxidant and immune functions of broilers challenged with lipopolysaccharide

作　　者：王祖澳 周晗聪 闫素 贾润怡 王蕾[1,2] 赵迪[1,2] 李鹏[1,2] 丁斌鹰[1,2] WANG Zuao;ZHOU Hancong;YAN Su;JIA Runyi;WANG Lei;ZHAO Di;LI Peng;DING Binying(Hubei Key Laboratory of Animal Nutrition and Feed Science,Wuhan Polytechnic University,Wuhan,Hubei Province 430023,China;Engineering Research Center of Feed Protein Resources on Agricultural By-products,Ministry of Education,Wuhan Polytechnic University,Wuhan,Hubei Province 430023,China)

机构地区：[1]武汉轻工大学动物营养与饲料科学湖北省重点试验室,湖北武汉430023 [2]武汉轻工大学农副产品蛋白质饲料资源教育部工程研究中心,湖北武汉430023

出　　处：《中国饲料》2024年第15期57-62,共6页China Feed

基　　金：湖北省重点研发计划项目“多酚功能植物饲料配方研发”(2022BBA0014)。

摘　　要：为研究山花黄芩提取物(CAB)对脂多糖(LPS)刺激的肉鸡肠道形态、抗氧化及免疫功能的影响,试验将240只1日龄的爱拔益加(AA)肉雏鸡(雄性)随机分为3组,每组8个重复,每个重复10只鸡,试验为期21 d。分组如下:(1)对照组:饲喂基础日粮;(2)LPS处理组:饲喂基础日粮,并进行LPS注射;(3)LPS+CAB处理组:饲喂添加500 mg/kg CAB的基础饲粮,并进行LPS注射。于试验结束时,LPS处理组和LPS+CAB处理组的肉鸡进行腹腔注射LPS(1 mg/kg BW),对照组注射等量生理盐水,3 h后采样分析。结果显示:与对照组相比,LPS处理组血液中IgG、IgA的含量分别极显著提升了11.93%、20.47%(P<0.01);与LPS处理组相比,LPS+CAB处理组血液中IgA的含量极显著提升了10.64%(P<0.01);与对照组相比,LPS处理组脾脏中IL-1β、IL-10、IL-17、TNF-α的含量分别极显著提升了112.29%、79.12%、86.88%、181.13%(P<0.01);与LPS处理组相比,LPS+CAB处理组脾脏中IL-6、IL-10、IL-17、TGF-β的含量分别极显著提升了43.84%、79.61%、100.88%、74.60%(P<0.01),LPS+CAB处理组脾脏中IFN-γ、IL-1β的含量分别极显著降低了43.56%、20.77%(P<0.01);与对照组相比,LPS处理组血液中丙二醛(MDA)的含量显著提升了43.68%(P<0.05);与对照组相比,LPS处理组回肠绒毛高度显著降低了25.43%(P<0.05);与LPS处理组相比,LPS+CAB处理组回肠绒毛高度显著提升了15.90%(P<0.05)。综上所述,LPS刺激引起肉鸡氧化应激及免疫功能受损,在日粮中添加CAB可在一定程度上缓解因LPS刺激对肉鸡抗氧化与免疫功能造成的负面影响。This experiment aimed to investigate the effects of scutellaria baicalensis extract(CAB)on the intestinal morphology,antioxidant and immune functions in broilers challenged with lipopolysaccharide(LPS).A total of 2401-day-old healthy AA male broilers with uniform weight were randomly assigned to 3 treatment groups,with 8 replicates in each group,and 10 birds each.The animal trial lasted for 21 days.Birds in the control group were fed with the basic diet,broilers in the LPS treated group were fed with the basic diet and injected with LPS.Birds in the LPS+CAB group were fed with a basal diet supplemented with 500 mg/kg CAB and injected with LPS.At the end of the animal trial,broilers in the LPS treatment group and LPS+CAB treatment group were intraperitoneally injected with LPS(1 mg/kg BW),while the control group was injected with an equal amount of physiological saline.The samples were harvested 3 hours later.Results showed that compared with the control group,the levels of IgG and IgA in the blood of the LPS treated group were significantly increased by 11.93%and 20.47%,respectively(P<0.01);Compared with the LPS treatment group,the IgA content in the blood of the LPS+CAB treatment group was significantly increased by 10.64%(P<0.01);Compared with the control group,the contents of IL-1β,IL-10,IL-17 and TNF-αin the spleen of the LPS treated group were significantly increased by 112.29%,79.12%,86.88%and 181.13%,respectively(P<0.01);Compared with the LPS treatment group,the contents of IL-6,IL-10,IL-17 and TGF-βin the spleen of the LPS+CAB treatment group were significantly increased by 43.84%,79.61%,100.88%and 74.60%(P<0.01),respectively.The contents of IFN-γand IL-1βin the spleen of the LPS+CAB treatment group were significantly reduced by 43.56%and 20.77%(P<0.01),respectively;Compared with the control group,the content of MDA in the blood of the LPS treatment group significantly increased by 43.68%(P<0.05);Compared with the control group,the height of ileal villi in the LPS treated group was significantly redu

关 键 词：山花黄芩提取物 肉鸡 抗氧化功能 免疫功能 脂多糖 

分 类 号：S816.7[农业科学—饲料科学]