长链非编码RNA RP11-572P18.1对卵巢癌细胞增殖和侵袭的影响及作用机制实验研究  

Effect of long non-coding RNA RP11-572P18.1 on the proliferation and invasion of ovarian cancer cells and its mechanism

作　　者：冷小飞 张逸群 魏峰 李俊[2] 朱颛顼 LENG Xiaofei;ZHANG Yiqun;WEI Feng;LI Jun;ZHU Zhuanxu(Department of Obstetrics and Gynecology,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China)

机构地区：[1]湖北医药学院附属十堰市太和医院妇产科,湖北十堰442000 [2]复旦大学附属中山医院肿瘤科,上海200032

出　　处：《陕西医学杂志》2024年第8期1011-1015,1020,共6页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(81802596)。

摘　　要：目的:探讨长链非编码RNA(lncRNA)RP11-572P18.1对卵巢癌细胞增殖和侵袭的影响及其分子机制。方法:采用国际癌症基因组联盟(ICGC)数据库分析卵巢癌组织中RP11-572P18.1表达水平。实时荧光定量PCR(RT-qPCR)检测卵巢癌细胞系OC3、A2780、Caov-3、SK-OV-3、HO-8910中RP11-572P18.1表达水平,选取表达水平最低的细胞进行后续实验。将Caov-3细胞分为RP11-572P18.1组和NC组,分别转染RP11-572P18.1过表达质粒和对照质粒。MTT实验检测Caov-3细胞增殖能力,Transwell实验检测Caov-3细胞侵袭能力。双荧光素酶报告基因实验验证RP11-572P18.1和miR-205-5p的靶向关系。采用RT-qPCR检测Caov-3细胞miR-205-5p表达。采用免疫印迹法(Western blot)检测Caov-3细胞增殖蛋白[细胞周期蛋白B(Cyclin B)、细胞周期蛋白E(Cyclin E)]和侵袭蛋白[锌指E盒同源结合蛋白1(Zeb1)、转录因子(Twist)、β-连环蛋白(β-catenin)]表达。结果:与正常组织比较,卵巢癌组织中RP11-572P18.1表达降低(P<0.01)。与正常卵巢上皮细胞IOSE80比较,卵巢癌细胞系中RP11-572P18.1表达水平降低,且Caov-3细胞降低最显著(均P<0.05)。与NC组比较,RP11-572P18.1组Caov-3细胞增殖能力及侵袭数目降低(均P<0.05)。RP11-572P18.1与miR-205-5p存在靶向关系。与NC组比较,上调RP11-572P18.1可抑制Caov-3细胞miR-205-5p以及Cyclin B、Cyclin E、Zeb1、Twist和β-catenin蛋白表达(均P<0.05)。结论:RP11-572P18.1在卵巢癌组织和细胞系中表达降低,RP11-572P18.1通过下调miR-205-5p抑制卵巢癌细胞的增殖和侵袭。Objective:To investigate the effects of long non-coding RNA(lncRNA)RP11-572P18.1 on the proliferation and invasion of ovarian cancer cells and its molecular mechanism.Methods:The expression level of RP11-572P18.1 in ovarian cancer tissues was analyzed using the international cancer genome consortium(ICGC)database.RT-qPCR was used to detect the expression levels of RP11-572P18.1 in ovarian cancer cell lines OC3,A2780,Caov-3,SK-OV-3 and HO-8910,and the cells with the lowest expression level were selected for follow-up experiments.The Caov-3 cells were divided into NC group and RP11-572P18.1 group,which were transfected with control plasmid and RP11-572P18.1 overexpression plasmid,respectively.MTT assay was used to detect the proliferation ability of Caov-3 cells,and Transwell assay was used to detect the invasion ability of Caov-3 cells.Dual luciferase reporter gene assay verified the targeting relationship between RP11-572P18.1 and miR-205-5p.The expression of miR-205-5p in Caov-3 cells was detected by RT-qPCR.Western blot was used to detect the expressions of proliferation proteins Cyclin B and Cyclin E and invasion proteins human Zinc finger E-box-binding homeobox 1(Zeb1),Twist andβ-catenin in Caov-3 cells.Results:Compared with normal tissues,the expression of RP11-572P18.1 in ovarian cancer tissues was decreased(P<0.01).Compared with normal ovarian epithelial cells IOSE80,the expression of RP11-572P18.1 in ovarian cancer cell lines decreased,and the expression of Caov-3 cells decreased most significantly(all P<0.05).Compared with the NC group,the proliferation ability and invasion number of Caov-3 cells in the RP11-572P18.1 group decreased(all P<0.05).There was a targeting relationship between RP11-572P18.1 and miR-205-5p.Compared with the NC group,up-regulation of RP11-572P18.1 inhibited the expressions of miR-205-5p and Cyclin B,Cyclin E,Zeb1,Twist andβ-catenin proteins in Caov-3 cells(all P<0.05).Conclusion:The expression of RP11-572P18.1 is decreased in ovarian cancer tissues and cell lines,and it can inh

关 键 词：卵巢癌 长链非编码RNA RP11-572P18.1 微小RNA-205-5p 细胞增殖 细胞侵袭 

分 类 号：R36[医药卫生—病理学]