结肠癌组织微小RNA-3607-3p、细胞周期蛋白E2表达及其与患者术后5年预后关系研究  

Expression of microRNA-3607-3p and cyclin E2 in colon cancer tissues and their relationship with 5-year prognosis of patients after surgery

作　　者：王瑜崴 李濛 康博 杨超 WANG Yuwei;LI Meng;KANG Bo;YANG Chao(First Department of Abdominal Surgery,Mudanjiang Tumor Hospital,Mudanjiang 157009,China)

机构地区：[1]牡丹江市肿瘤医院腹外一科,黑龙江牡丹江157009 [2]牡丹江市肿瘤医院放疗二科,黑龙江牡丹江157009 [3]牡丹江市肿瘤医院肿瘤内一科,黑龙江牡丹江157009 [4]牡丹江医学院附属第二医院康复医学科,黑龙江牡丹江157011

出　　处：《陕西医学杂志》2024年第8期1124-1129,共6页Shaanxi Medical Journal

基　　金：黑龙江省卫生健康委员会科研课题(20210505020120)。

摘　　要：目的:探讨结肠癌组织微小RNA-3607-3p(miR-3607-3p)、细胞周期蛋白E2(CCNE2)表达及其与患者术后5年预后的关系。方法:选取行结肠癌根治术治疗的88例结肠癌患者癌组织和癌旁组织。RT-qPCR法测定组织miR-3607-3p、CCNE2 mRNA表达。免疫组化法检测组织CCNE2蛋白表达。随访术后5年内结肠癌患者生存情况,根据生存情况将患者分为生存组(48例)和病死组(40例)。分析miR-3607-3p、CCNE2 mRNA与TNM分期、肿瘤分化程度、淋巴结转移的相关性。Cox回归分析结肠癌患者术后5年预后的影响因素。绘制受试者工作特征(ROC)曲线分析miR-3607-3p、CCNE2 mRNA对患者术后5年内生存的预测价值。结果:结肠癌组织miR-3607-3p表达水平低于癌旁组织,CCNE2 mRNA表达水平和CCNE2蛋白阳性表达率高于癌旁组织(均P<0.05)。miR-3607-3p低表达和CCNE2 mRNA高表达患者TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移的占比更高(均P<0.05)。结肠癌组织miR-3607-3p与CCNE2 mRNA、TNM分期、淋巴结转移呈负相关,与肿瘤分化程度呈正相关(均P<0.05)。CCNE2 mRNA与TNM分期、淋巴结转移呈正相关,与肿瘤分化程度呈负相关(均P<0.05)。miR-3607-3p高表达、CCNE2 mRNA低表达患者5年总生存率高于miR-3607-3p低表达、CCNE2 mRNA高表达患者(均P<0.05)。病死组TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移的患者占比更高(均P<0.05)。病死组结肠癌组织CCNE2 mRNA和蛋白阳性表达率高于生存组,miR-3607-3p低于生存组(均P<0.05)。TNM分期Ⅲ期、肿瘤低分化、有淋巴结转移、miR-3607-3p低表达、CCNE2 mRNA高表达、CCNE2蛋白阳性表达是影响结肠癌患者术后5年生存的独立危险因素(均P<0.05)。结肠癌组织miR-3607-3p、CCNE2 mRNA两者联合预测患者术后5年内生存的曲线下面积(AUC)高于miR-3607-3p、CCNE2 mRNA单独预测的AUC(均P<0.05)。结论:结肠癌组织miR-3607-3p呈低表达,CCNE2呈高表达,两者与患者术后5年预后有关,有望�Objective:To investigate the expression of microRNA-3607-3p(miR-3607-3p)and cyclin E2(CCNE2)in colon cancer tissues and their relationship with the 5-year prognosis of patients after surgery.Methods:The cancer tissues and paracancerous tissues of 88 colon cancer patients who underwent radical colon cancer resection were selected.The mRNA expressions of miR-3607-3p and CCNE2 in tissues were determined by RT-qPCR.Immunohistochemistry was used to detect the expression of CCNE2 protein in tissues.The survival of colon cancer patients within 5 years after surgery was followed-up,and the patients were divided into survival group(48 cases)and death group(40 cases)according to the survival situation.The correlation between miR-3607-3p and CCNE2 mRNA and TNM stage,tumor differentiation and lymph node metastasis was analyzed.Cox regression analysis was performed to analyze the influencing factors of 5-year prognosis of patients with colon cancer after surgery.The receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of miR-3607-3p and CCNE2 mRNA for the survival of patients within 5 years after surgery.Results:The expression level of miR-3607-3p in colon cancer tissues was lower than that in paracancerous tissues,and the expression level of CCNE2 mRNA and the positive expression rate of CCNE2 protein were higher than those in paracancerous tissues(all P<0.05).Patients with low miR-3607-3p expression and high CCNE2 mRNA expression had higher proportions of TNM stageⅢ,poor tumor differentiation and lymph node metastasis(all P<0.05).MiR-3607-3p in colon cancer tissues was negatively correlated with CCNE2 MRNA,TNM stage and lymph node metastasis,and positively correlated with tumor differentiation degree(all P<0.05).CCNE2 mRNA was positively correlated with TNM stage and lymph node metastasis,and negatively correlated with tumor differentiation degree(all P<0.05).The 5-year overall survival rate of patients with high miR-3607-3p expression and low CCNE2 mRNA expression was higher than that of

关 键 词：结肠癌 微小RNA-3607-3p 细胞周期蛋白E2 临床病理特征 相关性 预后 预测价值 

分 类 号：R735.35[医药卫生—肿瘤]