万山汞矿区弹琴蛙重金属富集与组织关联分析  

Analysis of Heavy Metal Bioaccumulation and Correlation in Nidirana adenopleura from the Wanshan Mercury Mining Area

作　　者：张晓东 王婷 卢志宏 钟莉传 陈芬 ZHANG Xiaodong;WANG Ting;LU Zhihong;ZHONG Lichuan;CHEN Fen(Guizhou Provincial Key Laboratory for Biodiversity Conservation and Utilization in the Fanjing Mountain Region,Tongren University,Tongren,554300,China;Guangxi Agricultural Vocational University,Nanning,530001,China)

机构地区：[1]铜仁学院贵州省梵净山地区生物多样性保护与利用重点实验室,铜仁554300 [2]广西农业职业技术大学,南宁530001

出　　处：《野生动物学报》2024年第3期596-606,共11页CHINESE JOURNAL OF WILDLIFE

基　　金：铜仁市科技计划项目(铜市科研[2020]127号);铜仁学院博士科研启动基金项目(trxyDH1909);贵州省科技计划项目(黔科合基础-ZK[2023]一般471)。

摘　　要：为了解万山汞矿区弹琴蛙(Nidirana adenopleura)组织重金属富集特征及其关联,对万山汞矿区典型农田生境内弹琴蛙组织(肝脏、皮肤、股骨、肌肉和趾骨)中铬(Cr)、锰(Mn)、镍(Ni)、铜(Cu)、锌(Zn)、镉(Cd)、铅(Pb)、砷(As)、硒(Se)和汞(Hg)这10种重金属元素的生物富集特征差异进行分析,结合非负矩阵分解分析法识别重金属元素的来源及贡献,并通过灰色关联度分析法分析弹琴蛙组织重金属元素间的关联性。结果表明:重金属元素在万山汞矿区弹琴蛙体内富集程度不同,平均质量分数由大到小依次为Zn、Cu、Mn、Pb、Se、Cr、Hg、Cd、Ni、As;Cu、Se、Cr、Ni、Cd和Hg主要富集在肝脏组织中,Mn、Zn和Pb主要富集在股骨和趾骨组织中,As主要富集在肌肉组织中。基于非负矩阵分解和相关分析,Hg、Cu、Cr、Pb和Se主要源于汞矿开采,As主要来源于农业活动,Mn主要来源于锰矿冶炼,Ni、Zn和Cd来源于多种复合途径。总之,两栖动物趾骨具有较好的生物指示作用,可作为一种非致命性的检测方法,用于动物重金属污染的研究。研究结果可为万山汞矿区环境污染防控和野生动物保护提供科学依据。To understand the heavy metal enrichment characteristics of the tissues of Nidirana adenopleura in the Wan⁃shan mercury mining area and their correlation,we analyzed the differences in the bioaccumulation characteristics of 10 heavy metal elements,namely Cr,Mn,Ni,Cu,Zn,Cd,Pb,As,Se,and Hg,in tissues of the N.adenopleura(liver,skin,femur,muscle,and phalanges)within the typical farmland habitats of the Wanshan mercury mining area.The sources and contributions of heavy metal elements were identified by nonnegative matrix factorization analysis,and the cor⁃relation between heavy metal elements in the tissues of N.adenopleura was analyzed by grey correlation analysis.The re⁃sults showed that the enrichment degree of heavy metal elements in the body of N.adenopleura in Wanshan mercury min⁃ing area was different.The average mass fraction was Zn,Cu,Mn,Pb,Se,Cr,Hg,Cd,Ni,and As in decending order.Cu,Se,Cr,Ni,Cd and Hg were mainly enriched in liver tissue,Mn,Zn and Pb in femur and phalanges,and As in muscle tissue.Based on the nonnegative matrix factorization and correlation analyses,Hg,Cu,Cr,Pb and Se originate mainly from mercury mining activities,As mainly from agricultural activities,Mn mainly from manganese mine smelting activities,and Ni,Zn and Cd from multiple composite pathways.In conclusion,amphibian phalanges has a good bioindicator func⁃tion,and can be used as a non-lethal assay for the study of animal heavy metal contamination.The results of this study can provide a scientific basis for the prevention and control of environmental pollution and wildlife protection in the Wanshan mer⁃cury mining area.

关 键 词：重金属 生物富集 弹琴蛙 汞矿 

分 类 号：Q89[生物学] X171[环境科学与工程—环境科学]