DGAT1在腮腺腺淋巴瘤组织中的表达及其临床意义  

Application of DGAT1 as a marker for clinical diagnosis of parotid adenolymphomas

作　　者：乔雪 白杨 张美丽 闫茹茜 窦林波 张彦聪 Qiao Xue;Bai Yang;Zhang Meili;Yan Ruxi;Dou Linbo;Zhang Yancong(School of Stomatology,Shandong Second Medical University,Weifang 261000,Shandong,China;Department of Pathology,Shandong University Qilu Hospital Dezhou Hospital(Dezhou People's Hospital),Dezhou 253000,Shandong,China;Department of Stomatology,Shandong University Qilu Hospital Dezhou Hospital(Dezhou People's Hospital),Dezhou 253000,Shandong,China)

机构地区：[1]山东第二医科大学口腔医学院,山东潍坊261000 [2]山东大学齐鲁医院德州医院(德州市人民医院)病理科,山东德州253000 [3]山东大学齐鲁医院德州医院(德州市人民医院)口腔科,山东德州253000

出　　处：《兰州大学学报（医学版）》2024年第6期22-28,共7页Journal of Lanzhou University（Medical Sciences）

摘　　要：目的 探讨二酰甘油O-酰基转移酶1 (DGAT1)在腮腺腺淋巴瘤组织中的表达及其临床意义。方法 回顾性分析山东大学齐鲁医院德州医院2012年1月—2021年12月腮腺腺淋巴瘤患者的临床资料,包括年龄、性别、单双侧发病、有无吸烟史、肿瘤位置、肿瘤直径和血脂水平。分别选取腮腺腺淋巴瘤、多形性腺瘤和正常腮腺的组织标本各5例,通过逆转录定量聚合酶链式反应(RT-qPCR)、蛋白质印迹法检测DGAT1在各组织中的表达情况。选取30例患者的病理切片免疫组织化学染色,检测DGAT1在腮腺腺淋巴瘤和正常腮腺组织中的表达差异。结果 111例腮腺腺淋巴瘤患者的年龄为25~84岁,平均年龄为(62.08±10.61)岁;男性92例(82.9%);有吸烟史者79例(71.2%),吸烟时间8~50年;单侧发病者103例(92.8%);肿瘤位于腮腺尾部者62例(55.9%);肿瘤直径为0.5~6.2 cm,平均直径为(2.998±1.083) cm,肿瘤直径为2.0~4.0 cm者76例(68.5%)。RT-qPCR、蛋白质印迹法结果显示DGAT1在腮腺腺淋巴瘤表达显著高于多形性腺瘤和正常腮腺组织。免疫组织化学结果显示DGAT1在腮腺腺淋巴瘤中高表达。结论 腮腺腺淋巴瘤多发于吸烟的中老年男性,病变位于腮腺后下极居多,肿瘤直径多为2.0~4.0 cm。DGAT1在腮腺腺淋巴瘤中高表达,显著高于正常腺体组织,提示DGAT1高表达与腮腺腺淋巴瘤发生密切相关,有望成为腮腺腺淋巴瘤诊断的新型标志物。Objective To investigate the expression and clinical significance of diglycerol O-acyltransferase 1(DGAT1) in parotid adenolymphomas tissue.Methods Clinical data of adenolymphoma patients from Shandong University Qilu Hospital Dezhou Hospital from January 2012 to December 2021 were collected,and their age,gender,unilateral and bilateral onset,smoking,tumor location and size,and blood lipids of the patients analyzed.Samples of parotid adenolymphomas,pleomorphic adenoma,and normal parotid tissue,each from 5 cases,were selected.The expression of DGAT1 in each tissue was detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR) and Western blotting.Immunohistochemical staining was performed on pathological sections of 30 patients to detect the expression differences of DGAT1in parotid adenolymphomas and normal parotid tissue.Results The ages of 111 patients with parotid adenolymphomas ranged from 25 to 84 years,with an average age of(62.08 ± 10.61) years;92 were male(82.9%);79 had a smoking history(71.2%),with smoking duration ranging from 8 to 50 years;103 had unilateral disease(92.8%);62 had tumors located at the tail of the parotid gland(55.9%);tumor diameters ranged from 0.5 to 6.2 cm,with an average diameter of(2.998 ± 1.083) cm;76 had tumors with diameters ranging from 2.0 to 4.0 cm(68.5%).RT-qPCR and Western blotting results showed that the expression of DGAT1 in parotid adenolymphomas was significantly higher than that in pleomorphic adenoma and normal parotid tissue.Immunohistochemical results showed a high expression of DGAT1 in parotid adenolymphomas.Conclusion Adenoma is more common in middle-aged and elderly male smokers.The lesions are mostly located in the posterior lower pole of the parotid gland,and the tumor size is mostly 2.0~4.0 cm.DGAT1 is highly expressed in parotid adenolymphomas,significantly higher than in normal glandular tissue,indicating a close correlation between a high expression of DGAT1 and the occurrence of parotid adenolymphomas,which is expected to become

关 键 词：腮腺腺淋巴瘤 临床特点 二酰甘油O-酰基转移酶1 血脂异常 

分 类 号：R739.87[医药卫生—肿瘤]