柴胡疏肝汤治疗癫痫大鼠的效果及其对miR-34a表达的影响  

Effect of Chaihu Shugan Decoction for the treatment of rats with epilepsy and its influence on miR⁃34a expression

作　　者：罗少翔 何乾超[2] 高玉广[2] 陈甜 王丹辉 农凤兰 傅达恒 LUO Shaoxiang;HE Qianchao;GAO Yuguang;CHEN Tian;WANG Danhui;NONG Fenglan;FU Daheng(The First School of Clinical Medicine,Guangxi University of Chinese Medicine,Nanning 530000,Guangxi,China;Department of Encephalopathy,the First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning 530000,Guangxi,China)

机构地区：[1]广西中医药大学第一临床医学院,广西南宁市530000 [2]广西中医药大学第一附属医院脑病科,广西南宁市530000

出　　处：《广西医学》2024年第6期867-872,共6页Guangxi Medical Journal

基　　金：广西自然科学基金(2020GXNSFAA238007);广西中医药重点学科建设项目(GZXK‑Z‑20‑13)。

摘　　要：目的探讨柴胡疏肝汤治疗癫痫大鼠的效果及其对miR‑34a表达的影响。方法将42只大鼠随机分为癫痫组(n=15)、柴胡疏肝汤组(n=12)及空白组(n=15)。除空白组外,另两组采用腹腔注射氯化锂-盐酸匹罗卡品法构建癫痫大鼠模型。造模结束后,给予柴胡疏肝汤组大鼠灌胃2 mL/100 g柴胡疏肝汤,空白组、癫痫组给予等量生理盐水灌胃,均为1次/d,持续灌胃3周。造模结束后,采用microRNA微阵列芯片检测癫痫大鼠模型海马组织差异表达的miRNA。干预期间观察记录3组大鼠的癫痫发作情况。最后一次灌胃结束后采用实时荧光定量PCR检测3组大鼠海马组织miR‑34a表达水平。结果MicroRNA微阵列芯片测序分析从癫痫大鼠海马组织中共筛选出40个差异表达的miRNA,其中miR‑34a在癫痫大鼠模型海马组织中呈高表达。与癫痫组比较,柴胡疏肝汤组大鼠癫痫发作次数、发作持续时间减少(P<0.05)。与空白组比较,癫痫组、柴胡疏肝汤组大鼠模型海马组织中miR‑34a的表达水平升高(P<0.05);与癫痫组比较,柴胡疏肝汤组大鼠模型海马组织中miR‑34a的表达水平降低(P<0.05)。结论柴胡疏肝汤可能通过抑制癫痫大鼠模型海马组织中miR‑34a的表达而减少癫痫发作。Objective To explore the effect of Chaihu Shugan Decoction for the treatment of rats with epilepsy and its influence on miR‑34a expression.Methods A total of 42 rats were randomly assigned to epilepsy group(n=15),Chaihu Shugan Decoction group(n=12),or blank group(n=15).Except for the blank group,the epilepsy rat model was established on the epilepsy group and the Chaihu Shugan Decoction group by employing the method of intraperitoneal injection of lithium chloride-pilocarpine hydrochloride.After the end of modeling,rats in the Chaihu Shugan Decoction group received intragastric administration of 2 mL/100 g Chaihu Shugan Decoction,whereas the blank group and the epilepsy group received intragastric administration of equivalent normal saline.All rats were given continuous intragastric administration once a day for 3 weeks.After the end of modeling,the microRNA microarray was used to detect miRNA of differential expression in hippocampus tissues in rats model with epilepsy.The epileptic seizure of rats in 3 groups was observed and recorded during intervention.The expression of miR-34a in hippocampus tissues of the 3 groups was detected by employing the real-time fluorescent quantitative PCR after the last time of intragastric administration.Results The analysis of MicroRNA microarray sequencing revealed that a total of 40 miRNAs of differential expression were screened out from hippocampus tissues in rats with epilepsy,therein miR-34a was high-expressed in hippocampus tissues of rats model with epilepsy.Compared with the epilepsy group,the Chaihu Shugan Decoction group exhibited decreased frequency of epileptic seizure,and shorter seizure duration(P<0.05).Compared with the blank group,the epilepsy group and the Chaihu Shugan Decoction group yielded elevated miR-34a expression in hippocampus tissues of rats model(P<0.05).Compared with the epilepsy group,the Chaihu Shugan Decoction group interpreted decreased miR-34a expression in hippocampus tissues of rats model(P<0.05).Conclusion Chaihu Shugan Decoction may reduc

关 键 词：癫痫 柴胡疏肝汤 微小RNA‑34a 大鼠 

分 类 号：R742.1[医药卫生—神经病学与精神病学]