2020—2021年河南省洛阳市肯塔基沙门菌的耐药水平及分子流行病学特征  

Drug resistance and molecular epidemiology of Salmonella Kentucky in Luoyang,Henan,2020-2021

作　　者：路立立 炊慧霞[2] 李会 韩琳 杨晓燕 杨璐 兰子君 潘奕鸥 朱鑫 张秀丽[2] LU Lili;CUAN Huixia;LI Hui;HAN Lin;YANG Xiaoyan;YANG Lu;LAN Zijun;PAN Yiou;ZHU Xin;ZHANG Xiuli(Luoyang Center for Disease Control and Prevention,Luoyang,Henan 471000,China;Henan Center for Disease Control and Prevention)

机构地区：[1]洛阳市疾病预防控中心,河南洛阳471000 [2]河南省疾病预防控制中心

出　　处：《现代疾病预防控制》2024年第7期486-493,共8页MODERN DISEASE CONTROL AND PREVENTION

基　　金：河南省医学科技攻关计划(联合共建)项目(LHGJ20230637)。

摘　　要：目的研究河南省洛阳市肉鸡供应链与腹泻患者粪便中分离到的肯塔基沙门菌(Salmonella Kentucky)的耐药水平及流行特征,为制定肯塔基沙门菌防控策略提供科学依据。方法对2020—2021年洛阳市监测的19株肯塔基沙门菌,进行14种抗生素的药物敏感性试验,全基因组测序进行多位点序列分型和耐药基因识别,采用脉冲场凝胶电脉(PFGE)及全基因组分析菌株的分子流行病学特征。结果19株肯塔基沙门菌中的16株来源于肉鸡养殖与屠宰环节,3株来源于腹泻患者粪便。菌株对环丙沙星、庆大霉素的耐药率均为100.00%,对氨苄西林、四环素、氯霉素的耐药率均为94.74%,对头孢噻肟、阿奇霉素的耐药率分别为84.21%和68.42%。全基因组测序显示,19株肯塔基沙门菌均为ST198型,所有菌株发生染色体上喹诺酮耐药决定区(QRDR)gyrA和parC双突变,突变类型有gyrA(ASP87Asn、Ser83Phe),parC(Thr57Ser、Ser80Ile、Ser395Asn、Ala469Ser和Thr620Ala),52.63%的菌株检出质粒介导的耐药基因(PMQR)QnrS1,β-内酰胺类耐药基因以bla_(CTX-M-55)(68.42%)和bla_(TEM-1)(26.32%)为主,大环内酯类耐药基因mphA、Mrx检出率为78.95%和89.47%。耐药基因的广泛检出与其高耐药表型一致。PFGE聚类分析及全基因组系统进化树结果显示,鸡源与人源部分菌株高度同源。结论本市肉鸡供应链与腹泻患者粪便中检出的肯塔基沙门菌均为ST198型,耐药率高,并携带相关的耐药基因,鸡源与人源部分菌株同源性高,可能已造成散发病例感染,应加强多重耐药肯塔基沙门菌在禽类养殖业中的监测。Objective To investigate the drug resistance profile and molecular epidemiological characteristics of Salmonella Kentucky strains isolated from the feces of broiler supply chain and diarrhea patients in Luoyang City,Henan Province,so as to provide a scientific basis for the development of prevention and control strategies.Methods Drug susceptibility testing of 14antibiotics was conducted on 19 strains of Salmonella Kentucky from Luoyang City between 2020 and 2021.Whole genome sequencing was used for multi-locus sequence typing and identification of resistance genes.PFGE and whole genome were used for the molecular epidemiological analysis.Results Out of the 19 strains sixteen were from the broiler breeding and slaughtering process,and there were from the feces of diarrhea patients.All strains were 100.00%resistant to ciprofloxacin and gentamicin,with high resistant rate of 94.74%to ampicillin,tetracycline and chloramphenicol.Resistance rate to cefotaxime and azithromycin were 84.21%and 68.42%,respectively.All strains belonged to ST198,with double mutations of gyrA and parC in the quinolone resistance determining region(QRDR)on the chromosome.The types of mutations were gyrA(ASP87Asn,Ser83Phe),parC(Thr57Ser,Ser80Ile,Ser395Asn,Ala469Ser and Thr620Ala).Plasmid-mediated resistance gene(PMQR)QnrS1 was detected in 52.63%of the strains.β-lactam resistance genes were dominated by blaCTX-M-55(68.42%)and blaTEM-1(26.32%).The detection rates of macrolide resistance genes mphA and Mrx were in 78.95%and 89.47%of the strains,respectively.The high detection of resistance genes was consistent with their high resistance phenotypes.PFGE clustering analysis and genome-wide phylogenetic tree showed high homology between some chicken and human origin strains Conclusions The Salmonella Kentucky strains isolated are all ST198 type,exhibit high drug resistance,and carry relevant resistance genes.There is significant homology between some chicken and human strains,suggesting potential disseminated of infections.Enhanced monitoring of mu

关 键 词：肯塔基沙门菌 耐药试验 全基因组测序 分子分型 

分 类 号：R446[医药卫生—诊断学]