高糖对乳牙牙髓干细胞生长及成骨分化能力的影响  

Preliminary Exploration of the Effect of High Sugar on the Growth and Osteogenic Differentiation Ability of Stem Cells from Human Exfoliated Deciduous Teeth

作　　者：李夏宁 任文平 赵红宇[3] LI Xianing;REN Wenping;ZHAO Hongyu(Periodontics,SATH Hospital of Stomatology Henan University,Zhengzhou 450002,China;Endodontics,SATH Hospital of Stomatology Henan University,Zhengzhou 450002,China;Department of Stomatolagy,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450002,China)

机构地区：[1]河南大学赛思口腔医院牙周病科,河南郑州450002 [2]河南大学赛思口腔医院牙体牙髓病科,河南郑州450002 [3]郑州大学第一附属医院口腔医学中心,河南郑州450002

出　　处：《河南医学研究》2024年第14期2497-2502,共6页Henan Medical Research

基　　金：中国工程科技发展战略河南研究院2022年战略咨询研究项目(2022HENYB02)。

摘　　要：目的探讨高糖对乳牙牙髓干细胞(SHED)生长及成骨分化能力的影响。方法从河南大学赛思口腔医院口腔颌面外科收集拔除的6~8岁无牙体牙髓牙周疾病的健康儿童的滞留乳牙,体外培养获得SHED,实验分为5.5 mmol·L^(-1)低糖组及25 mmol·L^(-1)高糖组。用细胞计数CCK-8试剂盒(CCK-8)检测两组细胞的增殖速度。分别用含两种不同糖浓度的成骨矿化液诱导21 d后,行茜素红染色实验,实时荧光定量PCR(QPCR)技术检测骨向分化相关基因碱性磷酸酶(ALP)、Runt相关转录因子2(RUNX2)和骨钙素(OCN)、骨桥蛋白(OPN)表达的差异。结果CCK-8检测结果显示,培养2~6 d时高糖组的OD值小于低糖组(P<0.05);成骨诱导后,高糖组的钙化结节数量少于低糖组,且高糖组的RUNX-2、ALP、OCN和OPN的相对表达量低于低糖组(P<0.05)。结论高糖抑制SHED的生长及成骨分化能力。Objective To observe the effect of high glucose on growth and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHED)in vitro.Methods Retained deciduous teeth(healthy children’s ages ranging from 6 to 8,without dental and periodontal disease)were obtained in the oral and maxillofacial surgery of SATH Hospital of Stomatology Henan University.SHED were gained by cultivated in vitro.The cultured SHED were divided into 5.5 mmol·L^(-1)low glucose concentration group and 25 mmol·L^(-1)high glucose concentration group.The cellular viability of the two groups were detected with CCK-8,respectively.After osteoinduction,the expression of calcium deposition by alizarin red staining on day 21,osteogenic differentiation related genes,such as alkaline phosphatase(ALP),Runt-related transcription factor 2(RUNX-2),osteocalcin(OCN)and osteopontin(OPN)by real time fluorogenic quantitative PCR(QPCR)in the two groups were observed respectively.Results The growth of the high glucose group was lower than the physiological group on day 2-6(P<0.05).After osteoinduction,the number of calcified nodules in the high glucose group was less than that in the low glucose concentration group,and the expression of ALP,OPN,RUNX-2,OCN genes in high glucose group were lower than those in physiological group(P<0.05).Conclusion High glucose can inhibit SHED’s growth and osteogenic differentiation.

关 键 词：高糖 乳牙牙髓干细胞 体外培养 细胞生长 成骨诱导 成骨分化 

分 类 号：R781[医药卫生—口腔医学]