帕金森病模型小鼠皮质神经元树突棘变化观察  

Observation of changes in dendritic spines of cortical neurons in Parkinson disease model mice

作　　者：贾苗 吕一霖 关晶心 刘萱 时子乔 张玉梅 JIA Miao;LYU Yilin;GUAN Jingxin;LIU Xuan;SHI Ziqiao;ZHANG Yumei(College of Basic Medical Science,Shenyang Medical College,Liaoning Province,Shenyang110034,China)

机构地区：[1]沈阳医学院基础医学院,辽宁沈阳110034

出　　处：《中国当代医药》2024年第22期13-17,共5页China Modern Medicine

基　　金：辽宁省教育厅科学研究经费项目(LJKZ1147);沈阳医学院大学生科研课题(20229024)。

摘　　要：目的观察帕金森病(PD)小鼠模型初级运动皮质锥体神经元树突棘变化情况并探索其可能机制。方法采用1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)建立PD小鼠模型,行为学实验和免疫组化检测黑质多巴胺神经元损伤情况进行模型鉴定,高尔基染色法观察锥体神经元树突和树突棘变化,免疫组化分析血清诱导激酶(SNK)和树突棘相关的Rap特异性GTPase活化蛋白(SPAR)的变化情况。结果MPTP组小鼠行为学得分、多巴胺阳性神经元平均光密度和树突棘密度低于正常对照组,MPTP组小鼠锥体神经元树突棘长度高于正常对照组,差异有统计学意义(P<0.05);两组的神经元树突复杂度比较,差异无统计学意义(P>0.05)。结论MPTP模型小鼠皮质神经元树突棘受损,树突棘的改变可能与SNK和SPAR表达变化有关。Objective To observe the changes in dendritic spines of primary motor cortex pyramidal neurons in Parkinson disease(PD)mouse model and explore possible mechanisms.Methods PD model was established using 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP),and behavioral experiments and immunohistochemistry were used to identify the damage of dopamine-positive neurons in the substantia nigra.Golgi staining was used to observe the changes in dendrites and dendritic spines of pyramidal neurons.Immunohistochemical analysis revealed changes in serum induced kinases(SNK)and spine-associated Rap specific GTPase activation proteins(SPAR).Results The behavioral score,mean optical density of dopamine neurons and dendritic spine density in the MPTP group were lower than those in the normal control group,and the length of the dendritic spines in pyramidal neurons in the MPTP group was significantly higher than that in the normal control group,and the differences were statistically significant(P<0.05).There was no significant difference in the dendritic complexity of neurons between the two groups(P>0.05).Conclusion The dendritic spines of cortical neurons in MPTP mice were damaged,and the changes in dendritic spines may be related to changes in SNK and SPAR expression.

关 键 词：帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 树突棘 皮质 血清诱导激酶 树突棘相关的Rap特异性GTPase活化蛋白 

分 类 号：R742.5[医药卫生—神经病学与精神病学]