卷丹百合黄酮提取物抗氧化活性研究  

Study on antioxidant activity of Lilium lancifolium Thunb flavonoid extract

作　　者：吴梦思 金建宇 尚书游 阮军 李硕芳 王劲松[1] 李蓉[1] WU Mengsi;JIN Jianyu;SHANG Shuyou;RUAN Jun;LI Shuofang;WANG Jinsong;LI Rong(Hubei Engineering Research Center for Specialty Flowers Biological Breeding,Jing Chu University of Technology,Jingmen 448000,China;Hubei Jurui Biotechnology Co.Ltd.,Jingmen 431900,China)

机构地区：[1]特色花卉生物育种湖北省工程研究中心,荆楚理工学院,湖北荆门448000 [2]湖北聚瑞生物科技有限公司,湖北荆门431900

出　　处：《食品与发酵工业》2024年第15期163-169,共7页Food and Fermentation Industries

基　　金：特色花卉生物育种湖北省工程研究中心开发基金项目(2022YB001);中药活性成分提取及药食同源保健品关键技术开发项目(2022BEC031);湖北省重点研发计划项目(2022BCE057);湖北省中央引导地方科技发展专项(2022BGE262)。

摘　　要：该实验旨在研究卷丹百合黄酮提取物抗氧化活性。以化学法、秀丽隐杆线虫模型为评价方法,用自由基清除率、抗氧化酶活力及线虫寿命等为指标,探究卷丹百合黄酮化合物的抗氧化能力。结果表明,卷丹百合黄酮提取物在料液比为1:10(g:mL)时,抗氧化活性达到最大值,其中DPPH自由基清除率为83.46%,ABTS阳离子自由基清除率为73.26%。卷丹百合黄酮提取物能显著提高线虫体内抗氧化酶活力和谷胱甘肽含量,提高线虫抗氧化能力,起到延长线虫寿命的作用。经过卷丹百合黄酮提取物处理的线虫在氧化应激条件下平均存活时间能达到10 h。卷丹百合黄酮提取物具有良好的抗氧化活性,具备开发食品抗氧化剂或功能食品的潜力。The purpose of this experiment was to study the antioxidant activity of Lilium lancifolium Thunb flavonoid extract.The antioxidant capacity of flavonoids of Lilium lancifolium Thunb was explored by chemical method and Caenorhabditis elegans model,and free radical scavenging,antioxidant enzyme activity,and nematode lifespan were used as indicators.Results showed that the antioxidant activity of Lilium lancifolium Thunb flavonoid extract reached a maximum when the material-liquid ratio of the extract was 1:10(g:mL),among which the DPPH radical scavenging rate was 83.46%and the ABTS cationic radical scavenging rate was 73.26%.The extract of flavonoids of Lilium lancifolium Thunb could significantly increase the activity of antioxidant enzymes and glutathione content in nematodes with oxidative damage,improve the antioxidant capacity of nematodes,and prolong the life of nematodes.The average survival time of nematodes treated with flavonoid extract of Lilium lancifolium Thunb reached 10 h.Lilium lancifolium Thunb flavonoid extract has good antioxidant activity and has the potential to develop food antioxidants or functional foods.

关 键 词：卷丹百合 黄酮提取物 秀丽隐杆线虫 抗氧化活性 

分 类 号：R284[医药卫生—中药学]