管花肉苁蓉提取物的工艺优化及其活性评价  

Cistanche tubulosa(Schenk)Wight extract and evaluation of whitening activity

作　　者：林炎钧 宫晓庆 杨思敏 彭效明[1] 严晓强 管洁 居瑞军[1] LIN Yanjun;GONG Xiaoqing;YANG Simin;PENG Xiaoming;YAN Xiaoqiang;GUAN Jie;JU Ruijun(Beijing Key Laboratory of Enze Biomass Fine Chemicals,Beijing Institute of Petrochemical Technology,Beijing 102617,China)

机构地区：[1]北京石油化工学院,恩泽生物质精细化工北京市重点实验室,北京102617

出　　处：《食品与发酵工业》2024年第15期170-176,共7页Food and Fermentation Industries

基　　金：国家自然科学基金项目(81703453);北京市教委科技计划一般项目(KM201810017002,KM202210017011);北京石油化工学院交叉科研探索基金资助项目(BIPTCSF-19&21)。

摘　　要：该研究旨在利用响应面法优化管花肉苁蓉活性成分的提取工艺,并进一步对其抗氧化与美白活性进行研究。通过超声-微波法提取管花肉苁蓉中活性成分,以提取物中松果菊苷为指标成分,结合Box-Behnken响应面法优化提取工艺,得到提取物后通过测试DPPH自由基、ABTS阳离子自由基清除能力、总还原力对其进行体外抗氧化活性评价;通过CCK-8法、流式法检测小鼠黑色素瘤细胞(B16细胞)相关指标并评价其体外美白活性。结果表明,最优提取工艺条件为料液比1:16(g:mL),乙醇体积分数70%,超声功率160 W,超声时间6 min,该条件下提取物中松果菊苷含量为8.09%;管花肉苁蓉提取物在2 mg/mL下自由基清除能力与抗坏血酸相当,有较好的总还原能力;当管花肉苁蓉提取物质量浓度为50 mg/mL时,B16细胞内黑色素含量降至58.12%,酪氨酸活性的抑制率达到32.10%,当提取物质量浓度为100 mg/mL时,黑素小体转运量降至12.77%,能显著抑制酪氨酸酶活性、黑色素的生成及黑素小体转运。管花肉苁蓉提取物表现出显著的体外抗氧化能力以及较好的美白效果,作为天然抗氧化、美白原料具有潜在开发价值。This study aims to optimize the extraction process of Cistanche tubulosa active components by response surface and to further investigate its antioxidant and whitening properties.Active components in C.tubulosa were extracted using microwave ultrasonic extraction.The echinacoside present in the extract was used as the index component,and the extraction process was further optimized by the Box-Behnken method of response surfaces.The antioxidant activity in vitro was assessed after the extract was obtained by testing the scavenging capacities of DPPH free radicals and ABTS cationic radicals,and total reducing power.The CCK-8 method and flow cytometry were used to detect related indexes of murine melanoma cells(B16 cells)and to evaluate their whitening activity in vitro.According to the findings,the obtained optimum values of extraction conditions were as follows:A material-to-liquid ratio of 1:16,an ethanol concentration of 70%,an ultrasonic power of 160 W,and a 6-minute ultrasonic period.Echinacoside was present in the extract at a level of 8.09%under these circumstances.At a concentration of 2 mg/mL,C.tubulosa extract had better total reducing ability and was comparable to vitamin C in terms of its ability to scavenge free radicals.The amount of melanin in B16 cells decreased to 58.12%and the rate of tyrosine activity inhibition increased to 32.10%at a concentration of 50 mg/mL of C.tubulosa extract.The amount of melanosome transport decreased to 12.77%at a concentration of 100 mg/mL of C.tubulosa extract,which significantly inhibited tyrosinase activity,melanin production,and melanosome transport.In vitro testing of the C.tubulosa extract revealed significant antioxidant capacity as well as a favorable whitening effect.It has potential as a natural raw material for whitening and antioxidants.

关 键 词：管花肉苁蓉 抗氧化能力 美白 B16细胞 黑素小体转运 

分 类 号：R284.2[医药卫生—中药学]