Anle138b对oAβ_(1-42)诱导大鼠海马神经元线粒体功能损伤的影响  

Effect of Anle138b on oAβ_(1-42)-induced damage in mitochondrial function of rat hippocampal neurons

作　　者：张丽[1] 刘欣宇 马泽刚[1] ZHANG Li;LIU Xinyu;MA Zegang(Department of Physiology,School of Basic Medicine,Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学教研室,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期337-340,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(81771437)。

摘　　要：目的探究Anle138b对慢性β淀粉样蛋白(Aβ)诱导的海马神经元线粒体功能损伤的影响。方法取出生24 h以内的SD乳鼠,原代培养海马神经元,待细胞成熟将其分为对照组(不进行任何处理)、oAβ_(1-42)组(100 nmol/L oAβ_(1-42)处理7 d)、oAβ_(1-42)+Anle138b组(100 nmol/L oAβ_(1-42)+100 nmol/L Anle138b共处理7 d)、Anle138b组(100 nmol/L Anle138b处理7 d)。各组处理结束后,应用流式细胞术检测活性氧(ROS)和线粒体膜电位(Δψm)阳性细胞的变化。结果析因设计的方差分析显示,oAβ_(1-42)和Anle138b的主效应明显(F_(oAβ1-42)=14.149、38.209,F_(Anle138b)=1.942、24.871,P<0.01),oAβ_(1-42)和Anle138b之间有交互性作用(F_(交互)=18.425、21.904,P<0.01)。单独效应分析显示,用oAβ_(1-42)处理时,Anle138b的处理效应明显(F=16.483、19.148,P<0.01);不用Anle138b处理时,oAβ_(1-42)的处理效应明显(F=14.149、38.209,P<0.01)。结论Anle138b可以减轻oAβ_(1-42)引起的海马神经元氧化应激和线粒体损伤,抑制oAβ_(1-42)对海马神经元的损伤。Objective To explore the effect of Anle138b on mitochondrial dysfunction in hippocampal neurons chronicallyexposed toβ-amyloid oligomers(oAβ_(1-42)).Methods We divided mature primary hippocampal neurons fromneonatal Sprague-Dawley rats within 24 h after birth into control group(without any treatment),oAβ_(1-42)group(treated with 100 nmol/L oAβ_(1-42)for 7 d),oAβ_(1-42)+Anle138b group(co-treated with 100 nmol/L oAβ_(1-42)and 100 nmol/L Anle138b for 7 d),and Anle138b group(treated with 100 nmol/L Anle138b for 7 d).After treatment,changes in reactive oxygen species and mitochondrial membrane potential(Δψm)-positive cells were measured by flow cytometry.Results According to the factorial analysis of variance,oAβ_(1-42)and Anle138b had significant main effects(F oAβ_(1-42)=14.149,38.209;F Anle138b=1.942,24.871;P<0.01),and there was an interaction between oAβ_(1-42)and Anle138b(F interaction=18.425,21.904;P<0.01).The individual effect analysis showed that in the presence of oAβ_(1-42),Anle138b produced significant effects(F=16.483,19.148;P<0.01),and in the absence of Anle138b,oAβ_(1-42)produced significant effects(F=14.149,38.209;P<0.01).Conclusion Anle138b can reduce oAβ_(1-42)-induced oxidative stress and mitochondrial damage in hippocampal neurons,inhibiting the damage to hippocampal neurons caused by oAβ_(1-42).

关 键 词：阿尔茨海默病 淀粉样β肽类 Anle138b 海马 神经元 氧化应激 大鼠 SPRAGUE-DAWLEY 

分 类 号：R745.7[医药卫生—神经病学与精神病学] R341[医药卫生—临床医学]