枸橼酸铁铵对小鼠黑质多巴胺能神经元电活动影响  

Effect of ferric ammonium citrate on the electricalactivity of dopaminergic neurons in the substantia ingra of mice

作　　者：谢如意 修民霞 石丽敏 XIE Ruyi;XIU Minxia;SHI Limin(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期341-344,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32171132)。

摘　　要：目的 探讨枸橼酸铁铵(FAC)对小鼠黑质多巴胺能神经元电活动影响。方法 取出生15~20 d的C57BL/6小鼠制备脑片,应用全细胞膜片钳技术观察FAC对黑质多巴胺能神经元自发放电活动及膜电位的影响。结果 小鼠黑质脑片置于FAC溶液(100μmol/L)中5 min后,多巴胺能神经元自发放电频率明显降低(t=5.533,P<0.01)。10 min后,多巴胺能神经元自发放电频率则显著减慢(t=7.297,P<0.001)。神经元膜电位明显向超级化方向移动(t=4.227,P<0.01)。结论 FAC能够抑制正常小鼠黑质多巴胺能神经元的电活动。Objective To investigate the effect of ferric ammonium citrate(FAC)on the electrical activity of dopaminergic neurons in the substantia nigra of mice.Methods Brain slices were prepared from the C57BL/6 mice of 15-20 days of postnatal age,and the whole-cell patch clamp technique was used to observe the effect of FAC on the spontaneous discharge and membrane potential of substantia nigra dopaminergic neurons.Results After the brain slices of the substantia nigra of mice infiltrated with 100μmol/L FAC solution for 5 minutes,there was a significant reduction in the frequency of spontaneous discharge of dopaminergic neurons(t=5.533,P<0.01),and after 10 minutes,there was a significant reduction in the frequency of spontaneous discharge of dopaminergic neurons(t=7.297,P<0.001).The neuronal membrane potential moved towards hyperpolarization(t=4.227,P<0.01).Conclusion FAC can inhibit the electrical activity of dopaminergic neurons in the substantia nigra of normal C57BL/6 mice.

关 键 词：帕金森病 铁 多巴胺能神经元 膜片钳术 小鼠 近交C57BL 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R591.1[医药卫生—临床医学]