外侧下丘脑与黑质致密部多巴胺能神经元的纤维投射  

Fibrous projections of the lateral hypothalamus to dopaminergic neurons in the substantia nigra pars compacta

作　　者：潘雪凝 王潇娅 谢俊霞 徐华敏 PAN Xuening;WANG Xiaoya;XIE Junxia;XU Huamin(Department of Physiology and Pathophysiology,Medical College of Qingdao University,Qingdao 266071,China)

机构地区：[1]青岛大学医学部基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期359-363,共5页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(31871202)。

摘　　要：目的 研究黑质致密部(SNpc)多巴胺能神经元是否接受外侧下丘脑(LH)的纤维投射。方法 使用逆行示踪剂、转基因小鼠和顺向病毒示踪技术探究LH与SNpc的投射关系。在C57BL/6雄性小鼠(8周龄)的SNpc区注射逆行示踪剂霍乱毒素亚基-B结合荧光素488(CTB-488),1周后制备冷冻脑切片,应用Olympus VS120荧光显微镜观察LH脑区CTB-488阳性神经元。在TH-Cre转基因雄性小鼠(8周龄)的LH注射顺向跨突触病毒AAV-hSyn-Flp-WPRE-hGH,在SNpc注射AAV-hSyn-Con/Fon-EYFP-WPRE-hGH,3周后灌注取脑并切片,观察SNpc和纹状体(Str)区EYFP阳性细胞及纤维。结果 逆行示踪实验表明,CTB-488注射后可在LH区观察到CTB-488阳性神经元,提示LH可能是SNpc的上游脑区。顺向病毒追踪实验结果显示,在小鼠SNpc观察到明显的EYFP阳性神经元且在Str可观察到EYFP阳性纤维,提示LH支配SNpc的多巴胺能神经元。结论 LH是SNpc的上游脑区,并支配SNpc的多巴胺能神经元。Objective To investigate whether dopaminergic neurons in the substantia nigra pars compacta(SNpc)can receive fibrous projections from the lateral hypothalamus(LH).Methods The techniques of retrograde tracers,transgenic mice,and anterograde neural circuit tracers were used to investigate the projection from the LH to the SNpc.The retrograde tracer cho-lera toxin subunit B(recombinant)Alexa Fluor TM 488 conjugate(CTB-488)was injected into the SNpc of male C57BL/6 mice aged 8 weeks;one week later,the brain was collected to prepare frozen brain slices,and the Olympus VS120 fluorescence microscope was used to observe CTB-488-positive neurons in the LH.The anterograde transneuronal virus AAV-hSyn-Flp-WPRE-hGH was injected into the LH of male TH-Cre transgenic mice aged 8 weeks,and AAV-hSyn-Con/Fon-EYFP-WPRE-hGH was injected into the SNpc of the mice;three weeks later,the brain was collected to prepare brain slices and observe EYFP-positive cells and fibers in the SNpc and the striatum(Str).Results The retrograde tracer experiment showed that CTB-488-positive neurons were observed in the LH after the injection of CTB-488,indicating that the LH might be the upstream brain region of the SNpc.The anterograde transneuronal virus tracking experiment showed that EYFP-positive neurons were observed in the SNpc of mice and EYFP-positive nerve fibers were observed in the Str,suggesting that the LH might dominate the dopaminergic neurons in the SNpc.Conclusion The LH is the upstream brain region of the SNpc and may dominate the dopaminergic neurons in the SNpc.

关 键 词：帕金森病 黑质 下丘脑区 侧 多巴胺能神经元 神经纤维 小鼠 转基因 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R322.81[医药卫生—临床医学]