MPTP慢性帕金森病模型小鼠步态的改变  

Gait changes in a mouse model of chronic Parkinson’s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine

作　　者：修民霞 谢如意 谢俊霞[1] 石丽敏[1] XIU Min-xia;XIE Ruyi;XIE Junxia;SHI Limin(Department of Physiology and Pathophysiology,School of Basic Medicine,Qingdao University Medical College,Qingdao 266071,China)

机构地区：[1]青岛大学基础医学院生理学与病理生理学系,山东青岛266071

出　　处：《青岛大学学报（医学版）》2024年第3期364-367,共4页Journal of Qingdao University(Medical Sciences)

基　　金：国家自然科学基金面上项目(32171132)。

摘　　要：目的 探究1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)慢性帕金森病(PD)模型小鼠步态改变。方法 模型组8周龄雄性C57BL/6小鼠给予腹腔注射MPTP(30 mg/kg体质量,每周2次,共4周),对照组注射等量的生理盐水,每组10只。采用动物步态分析系统(CatWalk)分别检测两组注射后1、2、3和4周小鼠步态相关指标。结果 步态分析结果显示,与对照组小鼠相比,模型组的运动持续时间、站立时间、脚步周期显著增加,平均速度、身体速度显著降低,差异均有统计学意义(t=2.209~5.176,P<0.05)。提示小鼠的步态发生改变。结论 MPTP慢性模型可以引起小鼠步态变化。Objective To investigate gait changes in a mouse model of chronic Parkinson’s disease(PD)induced by 1-met-hyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Methods Male C57BL/6 mice,aged 8 weeks,were intraperitoneally injec-ted with MPTP(30 mg/kg)twice a week for 4 weeks,and the mice in the control group were injected with an equal volumeof normal saline,with 10 mice in each group.The animal gait analysis system(CatWalk)was used to measure gait-related parameters at 1,2,3,and 4 weeks after injection.Results The results of gait analysis showed that compared with the control group,the model group had significant increases in run duration,stance,and step cycle and significant reductions in average speed and body speed(t=2.209-5.176,P<0.05).These results suggested gait changes in mice.Conclusion Gait changes are observed in a mouse model of chronic PD induced by MPTP.

关 键 词：帕金森病 1-甲基-4-苯基-1 2 3 6-四氢吡啶 步态分析 模型 动物 小鼠 近交C57BL 

分 类 号：R742.5[医药卫生—神经病学与精神病学] R442.9[医药卫生—临床医学]