Plackett-Burman设计法优化牡丹皮中丹皮酚的提取及包合工艺  

Optimization of Extraction and Inclusion Process of Paeonol from Moutan Peel by Plackett Burman Design

作　　者：张婷 邹献亮 邓钢 张园 ZHANG Ting;ZOU Xian-Liang;DENG Gang;ZHANG Yuan(School of Pharmacy,Pingxiang Health Vocational College,Pingxiang 337000,China)

机构地区：[1]萍乡卫生职业学院药学院,江西萍乡337000

出　　处：《中国药物经济学》2024年第6期92-96,共5页China Journal of Pharmaceutical Economics

基　　金：2021年度江西省教育厅科学技术研究项目(GJJ216706)。

摘　　要：目的采用Plackett-Burman试验设计优化牡丹皮中丹皮酚的提取及包合工艺。方法以丹皮酚提取率为指标,星点设计-效应面法优选提取和包合工艺,以温浸时间、加水量、蒸馏时间为丹皮酚提取的考察因素,利用Design expert设计优化牡丹皮的提取工艺;采用饱和水溶液法,以丹皮酚包封率为主要考核指标,以β-环糊精与丹皮酚摩尔比、搅拌时间、包合温度为丹皮酚包合的考察因素,利用Design expert设计优化丹皮酚的β-环糊精包合工艺。结果运用Design expert 13.0软件响应面分析,确定最佳提取工艺为温浸时间1.68 h,加水量13.41倍,蒸馏时间3.28 h,在此条件下,回归模型预测的丹皮酚提取量的理论值可以达到78.81%。确定丹皮酚最佳包合工艺为β-环糊精与丹皮酚摩尔比0.91∶1,包合时间3.12 h,包合温度48.6 h,在此条件下,回归模型预测的丹皮酚包合率理论值可以达到59.34%。结论经由星点设计-效应面法构建的数学模型具有良好的预测能力,通过此方法优化后的牡丹皮中丹皮酚提取率和包封率显著提高。Objective To optimize the extraction and cladding process of koaypanol in peony peel by Plackett-Burman experimental design.Methods Taking the extraction rate of paeonol as an index,the extraction and encapsulation process was optimized by the star point design-effect surface method,and the extraction process of peony peel was optimized by the design expert design based on the investigation factors of temperature immersion time,water addition and distillation time for the extraction of paeonol.Using the saturated aqueous solution method,taking the encapsulation rate of koayptol as the main assessment index,taking the molar ratio of β-cyclodextrin and paeonol,stirring time and wrapping temperature as the investigation factors of paeonol adhesion,the design expert was used to design and optimize the β-cyclodextrin cladding process of paeonol.Results Using the response surface analysis of Design expert 13.0 software,it was determined that the optimal extraction process was 1.68 h immersion time,13.41 times water addition,and distillation time 3.28 h,under which the theoretical value of paeonol extraction predicted by the regression model could reach 78.81%.The optimal cladding process of paeonol was determined to be a molar ratio of 0.91:1 between β-cyclodextrin and paeonpo,a cladding time of 3.12 h,and a cladding temperature of 48.6 h,under which the theoretical value of the cladding rate of dipamarine carboxyl predicted by the regression model could reach 59.34%.Conclusion The mathematical model constructed by the star point design-effect surface method has good predictive ability,and the extraction rate and encapsulation rate of paeonol in peony peel optimized by this method can be significantly improved.

关 键 词：Plackett-Burman试验设计 牡丹皮 丹皮酚 Β-环糊精 

分 类 号：R284.2[医药卫生—中药学]