一种复方中药精油抗菌消炎及驱蚊活性初步研究  

Preliminary study on antibacterial,anti⁃inflammatory,and mosquito repellent activities of a compound traditional Chinese medicine essential oil

作　　者：陈靖颖 杨晓旭 CHEN Jingying;YANG Xiaoxu(Department of Pharmacy,Harbin University of Commerce,Harbin,Heilongjiang 150001,China)

机构地区：[1]哈尔滨商业大学药学院,黑龙江哈尔滨150001

出　　处：《中华卫生杀虫药械》2024年第4期310-314,共5页Chinese Journal of Hygienic Insecticides and Equipments

基　　金：国家级大学生创新创业训练计划项目(202310240124X)。

摘　　要：目的评价一种复方中药精油的抑菌、抗炎活性及其驱蚊效果,并对其化学成分及相对含量进行分析。方法通过水蒸气蒸馏法提取复方精油;滤纸片法测试复方精油对金黄色葡萄球菌的抑菌活性;将36只小鼠随机分为正常组、模型组、丹皮酚软膏组及复方精油组,建立小鼠急性虫咬性皮炎模型,检测各组小鼠皮损处病理学切片及其炎症因子水平以验证复方精油对小鼠急性虫咬性皮炎的抗炎效果;对复方精油进行驱避蚊虫药效试验,验证其驱蚊效果;采用气相色谱-质谱联用技术(GC-MS)对复方精油进行定性和相对定量分析。结果与单方精油比较,复方精油对金黄色葡萄球菌生长的抑制效果更好,其抑菌圈直径为(17.25±0.37)mm,金黄色葡萄球菌对其对中度敏感;复方精油有效降低了小鼠虫咬性皮炎引起的皮肤组织水肿程度,减少了皮损组织炎性细胞产生与肥大细胞浸润,降低了小鼠血清TNF-α、IFN-γ、IL-6、IL-1β水平;复方精油对白纹伊蚊的有效驱避时间为7.83 h;GC-MS检测出精油中含有芳香类、萜类等28种化学成分。结论该复方精油有一定的抑制革兰氏阳性菌生长的作用,能够有效减轻小鼠虫咬性皮炎引起的皮肤炎症反应,有效成分中芳香类化合物占比最多,驱避时间优越,符合预期效果。Objective To investigate the antibacterial and antiflammatory activities of a traditional Chinese medicine compound essential oil,evaluate its mosquito repellent efficacy,and analyze the chemical constituents and their relative contents.Methods The compound essential oil was extracted by steam distillation;the antibacterial activity of the compound essential oil against Staphylococcus aureus was determined by kirby⁃Bauer test;36 mice were randomly divided into a normal group,a model group,a Danpi phenol ointment group and a compound essential oil group,the mouse model of acute insect bite dermatitis was established,the pathological sections and inflammatory factor levels of mouse skin lesions were tested to verify the anti⁃inflammatory effect on insect bite dermatitis.The qualitative and quantitative analysis of the compound essential oil was conducted by gas chromatography⁃mass spectrometer(GC⁃MS).The mosquito repellent pharmacodynamics experiment of compound essential oil was carried out to verify its mosquito repellent effect.Results Compared with single essential oil,compound essential oil had a better inhibitory effect on the growth of Staphylococcus aureus,with a diameter of(17.25±0.37)mm as the antibacterial zone,which was moderately sensitive to Staphylococcus aureus;The compound essential oil effectively reduced the degree of skin tissue edema caused by insect bite dermatitis in mice,reduced the production of inflammatory cells and infiltration of mast cells in skin lesions,and lowered the serum TNF⁃α,IFN⁃γ,IL⁃6,IL⁃1βlevel in mice;The effective repellent time of compound essential oil against Aedes albopictus was 7.83 hours.A total of 28 chemical components such as aromatics and terpenoids were detected in the compound essential oil.Conclusion The compound essential oil has an inhibitory effect on the growth of gram positive bacteria and can effectively alleviate the skin inflammatory response caused by insect bite dermatitis in mice.Aromatic compounds account for the highest proporti

关 键 词：驱蚊效果 复方精油 抑菌活性 消炎活性 

分 类 号：R184[医药卫生—流行病学]