温度和振动联合胁迫对花鲈有水保活运输中组织损伤及生化指标的影响  

Effects of temperature and vibration stress on tissue damage and biochemical indices in sea bass(Lateolabrax japonicus)during the process of keeping-alive

作　　者：郭美洁 梅俊 谢晶[1,2,3,4] GUO Meijie;MEI Jun;XIE Jing(College of Food Sciences and Technology,Shanghai Ocean University,Shanghai 201306,China;National Experimental Teaching Demonstration Center for Food Science and Engineering,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center of Aquatic Product Processing and Preservation,Shanghai Ocean University,Shanghai 201306,China;Shanghai Professional Technology Service Platform on Cold Chain Equipment Performance and Energy Saving Evaluation,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学食品学院,上海201306 [2]上海海洋大学,食品科学与工程国家级实验教学示范中心,上海201306 [3]上海海洋大学,上海市水产品加工保鲜工程技术研究中心,上海201306 [4]上海海洋大学,上海市冷链设备性能与节能评价专业技术服务平台,上海201306

出　　处：《水产学报》2024年第7期67-79,共13页Journal of Fisheries of China

基　　金：国家重点研发计划(2019YFD0901601);国家海水鱼产业技术体系(CARS-47-G26)。

摘　　要：为探究温度和振动强度联合胁迫在有水保活运输过程中对花鲈组织损伤及生化指标的影响,本研究以20和12℃的运输温度以及0、20和50 r/min的振动强度为变量,设置6个实验组,模拟运输48 h后,测定氧化应激、组织损伤和免疫反应等变化。结果显示:①温度对花鲈存活率的影响大于振动强度。运输24 h后,20℃的运输组,0、20、50 r/min的存活率分别为72.70%、62.50%和18.2%;而12℃各运输组存活率均为100%。②不同温度下各指标变化趋势差异明显。在20℃的运输条件下,过氧化氢酶(CAT)、谷胱甘肽-S-转移酶(GST)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LZM)活性和丙二醛(MDA)含量随着运输时间增加而升高,超氧化物歧化酶(SOD)和免疫球蛋白(IgM)含量随运输时间增加呈现先升高后下降的趋势,血糖(GLU)含量随着运输时间增加而下降。在12℃的运输温度下,CAT、MDA、ALT、AST、LZM呈现先上升后下降的趋势,SOD、GST、GLU、IgM活性呈现先下降后上升的趋势。20℃和50 r/min的运输组,SOD最高活性达0.64 U/mg prot,而12℃和50 r/min的运输组最高值仅有0.37 U/mg prot。研究表明,花鲈保活运输过程中应采用12℃的低温运输并尽量减少运输振动频率。本研究探索了温度和振动强度联合胁迫对海水鱼类组织损伤及生化指标的影响。研究结果可为花鲈短途运输提供参考依据。In order to explore the effects of combined stress of temperature and vibration intensity on tissue damage and biochemical indexes of sea bass(Lateolabrax japonicus)during in-water keep-alive transportation,six experimental groups were designed with transportation temperature of 20℃ and 12℃ and vibration intensity of 0,20 and 50 r/min as variables.After simulated transportation for 48 h,the effects of temperature and vibration stress on L.japonicus during simulated transportation were studied by analyzing oxidative stress,tissue damage and immune indexes.The results show that:①The vibration intensity of different temperature ratios has great influence on the survival rate of L.japonicus.After 24 h transportation,the survival rates of the transportation group at 20℃ at 0,20 and 50 r/min were 72.70,62.50 and 18.2%respectively.However,the survival rate of all transport groups at 12℃ is 100%.②The change trend of each index is different at different temperatures.Under the transport conditions of 20℃,catalase(CAT),glutathione-S-transferase(GST),malondialdehyde(MDA),glutamic acid transaminase(ALT),aspartate transaminase(AST),acid phosphatase(ACP),alkaline phosphatase(AKP),and lysozyme(LZM)increased with the increase of transport time,and superoxide dismutase(SOD),immunoglobulin(IgM)showed a tendency to increase and then decrease with increasing transport time,and blood glucose(GLU)showed a tendency to decrease with increasing transport time.Among them,low temperature treatment can reduce the oxidative stress,tissue damage and immune system damage of L.japonicus.At the transportation temperature of 12℃,the activities of CAT,MDA,ALT,AST and LZM increased first and then decreased,while the activities of SOD,GST,GLU and IgM decreased first and then increased.The highest value of SOD was 0.64 U/mgprot in the transportation groups at 20℃ and 50 r/min,but only 0.37 U/mgprot in the transportation group at 12℃ and 50 r/min.The research shows that the L.japonicus should be transported at low temperature,the te

关 键 词：花鲈 温度 振荡强度 氧化应激 组织损伤 免疫特性 

分 类 号：S981.14[农业科学—捕捞与储运]