一株高产L-缬氨酸菌株的诱变育种  

Mutation Breeding of a High-Yield L-Valine Strain

作　　者：王庆权[1] 刘海臣[2] 郭风 WANG Qingquan;LIU Haichen;GUO Feng(Jiangsu Bioactive Substances Engineering Research Center,School of Health Medicine,Jiangsu Food and Pharmaceutical Science College,Huai’an 223003,China;School of Chemical Engineering&Technology,China University of Mining and Technology,Xuzhou 221116,China;School of Pharmacy,Jiangsu Food and Pharmaceutical Science College,Huai’an 223003,China)

机构地区：[1]江苏食品药品职业技术学院健康医学院,江苏省生物活性物质工程研究中心,江苏淮安223003 [2]中国矿业大学化工学院,江苏徐州221116 [3]江苏食品药品职业技术学院药学院,江苏淮安223003

出　　处：《食品科技》2024年第6期11-18,共8页Food Science and Technology

基　　金：教育部中华酿酒传承与创新教学资源库项目(2022JXZYK001);江苏省高等学校基础科学(自然科学)研究面上项目(23KJD350002);江苏食品药品职业技术学院基金(自然科学)重点培育项目(302120220715)。

摘　　要：L-缬氨酸是一种人体必需氨基酸,可应用于食品领域。L-缬氨酸主要通过微生物发酵法生产,而高产菌株是发酵工业的核心。为获得能大量积累L-缬氨酸的发酵菌株,以黄色短杆菌(Brevibacterium flavum)FP-01为诱变出发菌株,经常压室温等离子体、紫外线、硫酸二乙酯和亚硝基胍的多次诱变处理,通过选育氨基酸结构类似物抗性与氨基酸营养缺陷突变株,获得一株L-缬氨酸的高产突变菌株B.flavum FP-6-56,该突变菌株摇瓶发酵的L-缬氨酸产量达54.5 g/L。遗传稳定性试验结果表明,突变菌株B.flavum FP-6-56的遗传标记和L-缬氨酸产量具有较强的稳定性。通过诱变育种获得了一株遗传稳定性高的L-缬氨酸高产突变菌株。L-valine is an essential amino acid.It is applied in food field.L-valine is mainly produced by microbial fermentation.High yielding strain is the foundation of the fermentation industry.Brevibacterium flavum FP-01 was mutated by atomspheric and room temperature plasma,ultra violet,diethyl sulfate,and nitrosoguanidine to breed mutants overproducing L-valine.A high yielding mutant strain,B.flavum FP-6-56,was obtained.It was resistant to amino acid structural analogues and was amino acid auxotrophic.54.5 g/L of L-valine was produced by the mutant strain in shaking flask.The results of genetic stability tests indicated that the genetic marker and L-valine production of B.flavum FP-6-56 were highly stable.A high-yield L-valine mutant strain with high genetic stability was obtained by mutation breeding.

关 键 词：L-缬氨酸 黄色短杆菌 诱变育种 氨基酸结构类似物 氨基酸营养缺陷 

分 类 号：TS201.3[轻工技术与工程—食品科学]