西太平洋深海水体惰性溶解有机碳降解菌群的细菌多样性  

Bacterial diversity of deep-sea water from the western Pacific Ocean enriched with recalcitrant dissolved organic carbon

作　　者：夏静宇 朱栩莹 王丽萍 邵宗泽 XIA Jingyu;ZHU Xuying;WANG Liping;SHAO Zongze(Key Laboratory of Marine Genetic Resources,Third Institute of Oceanography,MNR,Xiamen 361005,China)

机构地区：[1]自然资源部第三海洋研究所、自然资源部海洋生物遗传资源重点实验室,福建厦门361005

出　　处：《应用海洋学学报》2024年第3期419-428,共10页Journal of Applied Oceanography

基　　金：国家自然科学基金(42030412);中国大洋矿产资源研究开发协会项目(DY-XZ-04,DY135B01)。

摘　　要：深海水体微生物在有机物矿化中发挥着重要作用。惰性溶解有机碳(RDOC)在深海中最终归宿与微生物影响还有待研究。本研究利用富含RDOC的腐植酸和垃圾渗滤液为生长底物,对西太平洋6个站位不同深度(200~4700 m)水体样品的微生物进行了为期一年的实验室低温富集培养,结合高通量测序技术,对富集物中细菌多样性进行了分析。16S rRNA基因高通量测序结果表明,不同富集组的细菌组成相似,主要包括γ-变形菌纲的食烷菌属(Alcanivorax)、噬甲基菌属(Methylophaga)和Polycyclovorans属,α-变形菌纲的海旋菌属(Thalassospira)、副球菌属(Paracoccus)和短波单胞菌属(Brevundimonas),以及放线菌纲的类诺卡氏菌属(Nocardioides)等。这些优势微生物可能在原位参与了RDOC的深度矿化。Deep-sea microorganisms play an important role in organic matter mineralization.The ultimate fate and microbial impact of recalcitrant dissolved organic carbon(RDOC)in the deep sea still need to be further researched.In this study,microbes of water samples at different depths(200-4700 m)of 6 stations in the western Pacific Ocean were enriched with humic acid and landfill leachate at low temperature for one year,respectively.Bacterial diversity was analyzed using 16S rRNA gene high-throughput sequencing technology.Results showed that the bacterial compositions of enriched samples with different substrates were similar.The dominant genera contained Alcanivorax,Methylophaga and Polycyclovorans of Gammaproteobacteria,Thalassospira and Paracoccus of Alphaproteobacteria and Nocardioides of Actinobacteria.The dominant bacteria may participate in the deep mineralization of RDOC in situ.

关 键 词：海洋生物学 惰性溶解有机碳 矿化 微生物多样性 

分 类 号：P735[天文地球—海洋生物学]