红松子油皮诺敛酸靶向脂质合成关键酶HMGCR保护冠脉内皮细胞的作用研究  

Protective Effect of Pinolenic Acid in Pinus Koraiensis Seed Oil on Human Coronary Artery Endothelial Cells Injury Based on Pinolenic Acid Targets HMGCR,a Key Enzyme in Lipid Synthesis

作　　者：张壮 程梦娜 李娜[1] 石晓征[1] 曹占鸿 房星宇 刘健[1] 隋欣[1] ZHANG Zhuang;CHENG Mengna;LI Na;SHI Xiaozheng;CAO Zhanhong;FANG Xingyu;LIUJian;SUI Xin(Changchun University of Chinese Medicine,Changchun 130117,China;Jilin Agricultural Science and Technology University,Jilin 132101,China)

机构地区：[1]长春中医药大学,吉林长春130117 [2]吉林农业科技学院,吉林吉林132101

出　　处：《特产研究》2024年第4期91-97,共7页Special Wild Economic Animal and Plant Research

基　　金：长春中医药大学大学生创新创业训练计划项目(S202110199091);吉林省卫生与健康青年科技骨干培养计划项目(2020Q049)。

摘　　要：本研究主要围绕红松子油中的活性成分皮诺敛酸(PLA)靶向脂质合成关键酶HMGCR保护冠脉内皮细胞的作用进行探讨,并就其在动脉粥样硬化(AS)防治领域的应用作出理论指导。采用H2O2作用于HCAEC建立细胞损伤模型,将PLA和HMGCR进行分子对接,CCK8法检测细胞存活率,倒置显微镜观察HCAEC细胞形态,TC试剂盒测定HCAEC中TC含量,免疫荧光法及Western blot法检测HCAEC中HMGCR的表达水平。结果表明,PLA与HMGCR具有强相互作用,在PLA干预下细胞存活率有明显提高、细胞形态有较好改善、HCAEC中TC含量下降、HMGCR表达水平降低。PLA能够通过与HMGCR互作显著抑制HCAEC中HMGCR的表达,从而减少细胞内总胆固醇的水平,为冠脉内皮细胞损伤提供保护,为AS的预防和治疗提供理论依据。Pinus koraiensis seed oil extracted fromred pine seed is a natural vegetable oil,which contains a special unsaturated fatty acid-pinolenic acid.In this study,pinolenic acid,an active ingredient Pinus koraiensis seed oil,was used to target HMGCR,a key enzyme in lipid synthesis,to protect human coronary artery endothelial cells and its application in the prevention and treatment of AS was provided with theoretical guidance.HCAEC was treated with H2O2 to establish a cell damage model.PLAand HMGCR were moleculatively docked.Cytotoxicity was detected by the CCK8 method,morphological changes of cells were observed by microscope,and TC content in HCAECwas determined by TC kit.The expression level of HMGCRin HCAEC was detected by immunofluorescence andWestern blot.The results showed that PLA has strong interaction with HMGCR,and the survival rate and morphology of cells were significantly improved,TC content in HCAEC and HMGCR expression level were decreased under the intervention of PLA.PLA can significantly inhibit the expression of HMGCR in HCAEC by interacting with HMGCR,thus reducing the level of intracellular TC,providing protection for Human coronary artery endothelial cells injury,and providing theoretical basis for the prevention and treatment of AS.

关 键 词：红松子油 皮诺敛酸 总胆固醇 3-羟基-3-甲基戊二酸单酰辅酶A还原酶 分子对接 

分 类 号：R541.4[医药卫生—心血管疾病]