IL-6对种植体周围炎大鼠牙龈组织中TNF-α表达的影响  

Mechanisms underlying the influence of IL-6 on TNF-α expression in the gingival tissue of rats with peri-implantitis

作　　者：黄大海 王祥芸 储雯 胡蓉[1] 温红萍 HUANG Dahai;WANG Xiangyun;CHU Wen(The Affiliated Hospital of Yunnan University(Yunnan Second People’s Hospital),Yunnan,Kunming 650031,China)

机构地区：[1]云南大学附属医院云南省第二人民医院,昆明市650031

出　　处：《河北医药》2024年第15期2256-2260,共5页Hebei Medical Journal

基　　金：云南省科技厅科技计划项目(编号:202001BA070023-0317)。

摘　　要：目的 探究白细介素(IL-6)对种植体周围炎大鼠牙龈组织中肿瘤坏死因子-α(TNF-α)表达的机制。方法 按照随机数字表法将40只大鼠分为假手术组(sham组)、种植体周围炎大鼠模型组(PI组)、种植体周围炎大鼠模型给予IL-6抑制剂Tocilizumab组(Tocilizumab组)、在Tocilizumab组的基础上给予NF-κB/p65激活剂LPS组(LPS组),每组10只。观察种植体周围软组织袋深度(PPD)、探诊出血(BOP)、出血指数(BI)和牙龈指数(GI)。测量种植体周围骨高度和骨密度(Micro-CT法);检测种植体周围牙龈组织炎症浸润(HE染色);RT-PCR检测种植体周围牙龈组织中IL-6、TNF-α mRNA表达;检测牙龈组织中p-p65蛋白表达(蛋白质印迹法)。结果 与sham组比较,PI组大鼠种植体软组织中PPD(近颊、远颊、近舌、远舌)、BOP、BI和GI均明显增加(P<0.05);与PI组比较,Tocilizumab组大鼠种植体软组织中PPD(近颊、远颊、近舌、远舌)、BOP、BI和GI均明显降低(P<0.05);与Tocilizumab组比较,种植体软组织中PPD(近颊、远颊、近舌、远舌)、BOP、BI和GI均明显增加(P<0.05)。与sham组比较,PI组大鼠种植体骨高度、牙槽骨密度明显降低,IL-6、TNF-α mRNA和p-p65蛋白表达增加(P<0.05);与PI组比较,Tocilizumab组大鼠种植体骨高度、牙槽骨密度明显升高,IL-6、TNF-α mRNA和p-p65蛋白表达低于(P<0.05);LPS组大鼠种植体骨高度、牙槽骨密度低于Tocilizumab组,IL-6、TNF-α mRNA和p-p65蛋白表达高于Tocilizumab组(P<0.05)。结论 IL-6抑制剂可通过抑制牙髓组织中NF-κB通路激活,进而抑制种植体周围炎大鼠牙龈组织中TNF-α释放,从而减轻大鼠种植体周围炎发展。Objective To investigate the mechanism underlying the influence of interleukin-6(IL-6) on tumor necrosis factor-alpha(TNF-α) expression in the gingival tissue of rats with peri-implantitis.Methods Forty rats were randomly assigned into sham operation group(sham group),peri-implantitis group(PI group),PI + IL-6 inhibitor Tocilizumab group(Tocilizumab group),and PI + Tocilizumab + nuclear factor-kappa B(NF-κB)/p65 activator lipopolysaccharide(LPS) group(LPS group),with 10 rats in each group.The periodontal pocket depth(PPD) in peri-implant soft tissue,bleeding on probing(BOP),bleeding index(BI) and gingival index(GI) were detected.Peri-implant bone height and bone density were measured by Micro-CT.Inflammatory infiltration in peri-implant gingival tissue was observed by hematoxylin and eosin(H&E) staining.The mRNA levels of IL-6 and TNF-α in peri-implant gingival tissue were examined by real-time reverse transcription polymerase chain reaction(RT-PCR).Protein level of p-p65 in gingival tissue was examined by Western blot.Results Compared with those of the sham group,PPD(proximal cheek,distal cheek,proximal tongue,distal tongue),BOP,BI and GI were significantly higher in the peri-implant soft tissues of rats in the PI group(P<0.05).Compared with those of the PI group,PPD(proximal cheek,distal cheek,proximal tongue,distal tongue),BOP,BI and GI were significantly lower in the peri-implant soft tissues of rats in the Tocilizumab group(P<0.05).The PPD(proximal buccal,distal buccal,proximal tongue,distal tongue),BOP,BI and GI were significantly higher in the peri-implant soft tissues of LPS group compared with those of the Tocilizumab group(P<0.05).Compared with the sham group,the peri-implant bone height and alveolar bone density were significantly lower,and the mRNA levels of IL-6 and TNF-α and protein level of p-p65 were significantly higher in the PI group(P<0.05).Compared with those of the PI group,the peri-implant bone height and alveolar bone density were significantly higher,and the mRNA levels of IL-6 and TN

关 键 词：白细胞介素-6 种植体周围炎 TNF-Α NF-ΚB通路 

分 类 号：R782.1[医药卫生—口腔医学]