ETS1在肺腺癌组织中表达变化及对肺腺癌细胞增殖迁移侵袭能力的影响  

Expression changes of ETS1 in lung adenocarcinoma tissues and its effects on proliferation,migration,and invasion of lung adenocarcinoma cells

作　　者：李彬 张维莹 曹雪如[2] 王梅 LI Bin;ZHANG Weiying;CAO Xueru;WANG Mei(Department of Pulmonary and Critical Care Medicine,Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;不详)

机构地区：[1]山东第一医科大学附属省立医院呼吸与危重症医学科,济南250021 [2]菏泽市立医院呼吸与危重症医学科 [3]山东第一医科大学附属省立医院科研部

出　　处：《山东医药》2024年第23期21-25,共5页Shandong Medical Journal

摘　　要：目的探讨ETS1在肺腺癌发生发展中的作用,为肺腺癌临床预后标志物的筛选和靶向干预提供理论依据。方法利用UAlcan数据库访问癌症基因组图谱(TCGA)数据库和CPTAC数据库,比较肺腺癌组织和癌旁正常组织ETS1表达;根据TCGA数据库肺腺癌组织ETS1表达情况,比较ETS1高、低表达肺腺癌患者的总生存期(OS)。取对数生长期的人肺腺癌A549细胞,随机分为si-NC组、si-ETS1组、vector组、ETS1-OE组,分别转染si-NC、si-ETS1、pcDNA3.1空载质粒、pcDNA3.1-ETS1过表达质粒;采用Western blotting法和实时荧光定量PCR法检测ETS1蛋白、mRNA相对表达量,CCK-8法观察细胞培养0~96 h的增殖能力[以光密度(OD)值表示],细胞划痕实验观察细胞迁移能力(以划痕愈合率表示),Transwell小室实验观察细胞侵袭能力(以侵袭细胞数表示)。结果TCGA和CPTAC数据库分析结果均显示,肺腺癌组织ETS1表达低于癌旁正常组织(P均<0.05)。与ETS1低表达肺腺癌患者比较,ETS1高表达肺腺癌患者OS更长(P<0.05)。与si-NC组及vector组比较,si-ETS1组ETS1蛋白及mRNA相对表达量均降低,ETS1-OE组ETS1蛋白及mRNA相对表达量均升高(P均<0.05)。随着培养时间的延长,各组细胞OD值均呈升高趋势;与si-NC组及vector组同时间点比较,si-ETS1组细胞OD值均升高,ETS1-OE组细胞OD值均降低(P均<0.05)。与si-NC组及vector组同时间点比较,si-ETS1组细胞划痕愈合率、侵袭细胞数均升高,ETS1-OE组细胞划痕愈合率、侵袭细胞数均降低(P均<0.05)。结论肺腺癌组织ETS1表达降低且与患者预后有关,ETS1降表达可促进肺腺癌细胞的增殖、迁移及侵袭,有成为肺腺癌患者预后标志物及治疗靶基因的潜在价值。Objective To discuss the role of ETS1 in development and progression of lung adenocarcinoma and to provide a theoretical basis for the screening of clinical biomarkers and targeted intervention for lung adenocarcinoma.Methods The UAlcan database was used to access the Cancer Genome Atlas(TCGA)database and the CPTAC database to compare the expression of ETS1 in lung adenocarcinoma tissues and normal adjacent tissues.According to the expres⁃sion of ETS1 in lung adenocarcinoma tissues of the TCGA database,the overall survival(OS)of lung adenocarcinoma pa⁃tients with high and low ETS1 expression was compared.Human lung adenocarcinoma A549 cells in the logarithmic growth phase were randomly divided into the si-NC group,si-ETS1 group,vector group and ETS1-OE group,which were transfect⁃ed with si-NC,si-ETS1,pcDNA3.1 unloaded plasmid and pcDNA3.1-ETS1 overexpression plasmid by Lipofectamine™3000 transfection reagent,respectively.The relative expression of ETS1 protein and mRNA was detected by Western blot⁃ting and real-time quantitative PCR,the cell proliferation ability(expressed by optical density value)was observed by CCK-8,the cell migration ability(expressed by scratch healing rate)was observed by cell scratch assay,and the cell inva⁃sion ability(expressed by the number of invasive cells)was observed by Transwell chamber assay.Results The results of TCGA and CPTAC database analysis showed that the expression of ETS1 in lung adenocarcinoma tissues was lower than that in the normal adjacent tissues(P<0.05).Compared with patients with low ETS1 expression in lung adenocarcinoma,patients with high ETS1 expression had longer OS(P<0.05).Compared with the si-NC group and the vector group,the relative expression levels of ETS1 protein and mRNA in the si-ETS1 group decreased,and the relative expression levels of ETS1 protein and mRNA in the ETS1-OE group increased(all P<0.05).Over the time of culture,the OD value of cells in each group showed an increasing trend.Compared with the si-NC group and the vector group a

关 键 词：ETS1 肺腺癌 预后 细胞迁移 细胞侵袭 细胞增殖 

分 类 号：R734.2[医药卫生—肿瘤]