急性髓系白血病患者血清LncRNA PVT1、miR-486-5p表达及预后价值  

Expression and prognostic value of serum LncRNA PVT1,miR-486-5p in patients with acute myeloid leukocyte

作　　者：陈珊[1] 安和兵[1] 王腾飞 宗广帅 周蕾 Chen Shan;An Hebing;Wang Tengfei;Zong Guangshuai;Zhou Lei(Department of Clinical Laboratory,the Second Affiliated Hospital of Hebei North University,Hebei Province,Zhangjiakou 075100,China;不详)

机构地区：[1]河北北方学院附属第二医院检验科,河北张家口075100 [2]河北北方学院附属第二医院血液科,河北张家口075100

出　　处：《疑难病杂志》2024年第8期955-960,共6页Chinese Journal of Difficult and Complicated Cases

基　　金：河北省卫生健康委科研基金项目(20200492)。

摘　　要：目的探讨急性髓系白血病(AML)患者血清长链非编码RNA PVT1基因(LncRNA PVT1)、微小RNA-486-5p(miR-486-5p)表达水平与疾病病理特征和预后的关系。方法回顾性选取2017年4月—2019年3月河北北方学院附属第二医院血液科就诊的AML患者100例为AML组,选取同期健康体检者100例为健康对照组,采用实时荧光定量聚合酶链反应(RT-qPCR)检测血清LncRNA PVT1、miR-486-5p相对表达量;采用Pearson相关法分析AML患者血清LncRNA PVT1与miR-486-5p水平的相关性;Kaplan-Meier法分析AML患者生存曲线;采用Cox回归分析评价影响AML患者预后的危险因素。结果AML组患者LncRNA PVT1水平高于健康对照组,miR-486-5p水平低于健康对照组(t/P=15.403/<0.001、18.305/<0.001)。Pearson相关性分析显示,AML患者LncRNA PVT1与miR-486-5p水平呈负相关(r=-0.261,P<0.01);不同白细胞计数、骨髓原始细胞比率、NCCN危险分层患者LncRNA PVT1、miR-486-5p水平比较差异有统计学意义(LncRNA PVT1:t/P=2.934/0.004,2.901/0.005,10.985/<0.001;miR-486-5p:t/P=2.598/0.012,2.604/0.012,8.593/<0.001)。LncRNA PVT1高水平表达者3年总生存率低于LncRNA PVT1低水平表达者(χ^(2)/P=14.16/<0.001),miR-486-5p高水平表达者3年总生存率高于miR-486-5p低水平表达者(χ^(2)/P=16.82/<0.001)。Cox回归分析显示,LncRNA PVT1高表达、miR-486-5p低表达、NCCN危险分层高是急性髓系白血病患者预后不良的危险因素[RR(95%CI)=1.982(1.148~2.993),1.668(1.085~2.641),1.659(1.068~2.602)]。结论AML患者LncRNA PVT1高表达,miR-486-5p低表达,二者水平与白细胞计数、骨髓原始细胞比率、NCCN危险分层和患者预后有关。Objective To explore the relationship between the serum expression levels of long non-coding RNAPVT1 gene(LncRNAPVT1)and microRNA-486-5p(miR-486-5p)in plasma and the pathological characteristics and prognosis of acute myeloid leukemia(AML)patients.Methods One hundred patients with AML were selected as AML group and 100 healthy subjects were selected as healthy control group.The relative expression levels of serum LncRNA PVT1 and miR-486-5p were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Pearson correlation method was used to evaluate the correlation between serum LncRNA PVT1 and miR-486-5p in AML patients and its relationship with pathological characteristics.Kaplan-Meier method was used to analyze the survival curve of AML patients,and Cox regression analysis was used to evaluate the risk factors affecting the prognosis of AML patients.Results The levels of LncRNA PVT1 in AML experimental group patients were higher than those in the healthy control group.the levels of miR-486-5p in AML experimental group patients were lower than those in the healthy control group(t/P=15.403/<0.001、18.305/<0.001).Pearson correlation analysis showed that LncRNA PVT1 was negatively correlated with miR-486-5p level in AML group(r=-0.261,P<0.01).There were statistically significant differences in leukocyte count,bone marrow original cell ratio,prognostic risk stratification,LncRNA PVT1 and miR-486-5p levels in AML group(LncRNA PVT1:t/P=2.934/0.004,2.901/0.005,10.985/<0.001;miR-486-5p:t/P=2.598/0.012,2.604/0.012,8.593/<0.001).The 3-year overall survival rate of those with high expression of LncRNA PVT1 was lower than those with low expression of LncRNA PVT1(χ^(2)/P=14.160/<0.001),and the 3-year overall survival rate of those with high expression of miR-486-5p was higher than that of those with low expression of miR-486-5p(χ^(2)/P=16.820/<0.001).Comprehensive analysis of study data showed that high level of LncRNA PVT1 expression and low level of miR-486-5p expression were risk factors for po

关 键 词：急性髓系白血病 长链非编码RNA PVT1基因 微小RNA-486-5p 病理特征 预后 

分 类 号：R733.71[医药卫生—肿瘤]