Leech Poecilobdella manillensis protein extract ameliorated hyperuricemia by restoring gut microbiota dysregulation and affecting serum metabolites  

作　　者：Xia Liu Xing-Qiu Liang Tian-Cai Lu Zhe Feng Min Zhang Nan-Qing Liao Feng-Lian Zhang Bo Wang Li-Sheng Wang 

机构地区：[1]Medical College,Guangxi University,Nanning 530004,Guangxi Zhuang Autonomous Region,China [2]Department of Traditional Chinese Medicine,HIV/AIDS Clinical Treatment Center of Guangxi(Nanning),The Fourth People’s Hospital of Nanning,Nanning 530023,Guangxi Zhuang Autonomous Region,China [3]Department of Science and Technology,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,Guangxi Zhuang Autonomous Region,China [4]General Manager’s Office,Guangxi Fuxinyi Biological Technology Co.Ltd.,Pingnan 537300,Guangxi Zhuang Autonomous Region,China [5]Department of Joint and Sports Medicine,Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine,Nanning 530011,Guangxi Zhuang Autonomous Region,China [6]Department of Gerontology,Nanning Social Welfare Hospital,Nanning 530004,Guangxi Zhuang Autonomous Region,China

出　　处：《World Journal of Gastroenterology》2024年第29期3488-3510,共23页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China,No.82160843.

摘　　要：BACKGROUND Hyperuricemia(HUA)is a public health concern that needs to be solved urgently.The lyophilized powder of Poecilobdella manillensis has been shown to significantly alleviate HUA;however,its underlying metabolic regulation remains unclear.AIM To explore the underlying mechanisms of Poecilobdella manillensis in HUA based on modulation of the gut microbiota and host metabolism.METHODS A mouse model of rapid HUA was established using a high-purine diet and potassium oxonate injections.The mice received oral drugs or saline.Additionally,16S rRNA sequencing and ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry-based untargeted metabolomics were performed to identify changes in the microbiome and host metabolome,respectively.The levels of uric acid transporters and epithelial tight junction proteins in the renal and intestinal tissues were analyzed using an enzyme-linked immunosorbent assay.RESULTS The protein extract of Poecilobdella manillensis lyophilized powder(49 mg/kg)showed an enhanced anti-trioxypurine ability than that of allopurinol(5 mg/kg)(P<0.05).A total of nine bacterial genera were identified to be closely related to the anti-trioxypurine activity of Poecilobdella manillensis powder,which included the genera of Prevotella,Delftia,Dialister,Akkermansia,Lactococcus,Escherichia_Shigella,Enterococcus,and Bacteroides.Furthermore,22 metabolites in the serum were found to be closely related to the anti-trioxypurine activity of Poecilobdella manillensis powder,which correlated to the Kyoto Encyclopedia of Genes and Genomes pathways of cysteine and methionine metabolism,sphingolipid metabolism,galactose metabolism,and phenylalanine,tyrosine,and tryptophan biosynthesis.Correlation analysis found that changes in the gut microbiota were significantly related to these metabolites.CONCLUSION The proteins in Poecilobdella manillensis powder were effective for HUA.Mechanistically,they are associated with improvements in gut microbiota dysbiosis and the regulation of sp

关 键 词：Gut microbiota Metabolism Multi-omics Poecilobdella manillensis Sphingolipid metabolism pathway Galactose metabolism pathway HYPERURICEMIA 

分 类 号：R589.7[医药卫生—内分泌]