甘草多糖对LPS诱导的A549细胞炎症损伤的拮抗作用研究  

Antagonistic Effect of Glycyrrhiza Polysaccharides on LPS-induced Inflammation Damagein A549 Cells

作　　者：苏瑞军 杨琳雪 唐瑜 武晓英[1] SU Ruijun;YANG Linxue;TANG Yu;WU Xiaoying(College of Biological Sciences and Technology,Taiyuan Normal University,Jinzhong Shanxi 030619,China)

机构地区：[1]太原师范学院生物科学与技术学院,山西晋中030619

出　　处：《太原师范学院学报（自然科学版）》2024年第2期58-63,共6页Journal of Taiyuan Normal University:Natural Science Edition

基　　金：山西省基础研究计划自由探索类青年科学研究项目(20210302124587)。

摘　　要：甘草是世界上应用最广泛草本植物之一,其中主要提取物甘草多糖是具有多种生物活性的多糖类化合物.旨在探究甘草多糖(Glycyrrhiza polysaccharide)对脂多糖(LPS)诱导的A549细胞炎症的拮抗作用的分子机理,为治疗肺部炎症疾病提供一定理论依据.采用水提醇沉法提取甘草多糖粗提物.利用细胞增殖检测试剂盒CCK-8检测不同浓度甘草多糖以及LPS对A549细胞活力的影响;利用酶联免疫吸附法(ELISA)检测细胞内TNF-α和IL-6因子的表达情况;利用实时荧光定量PCR检测细胞内IL-6、IL-1β、NF-κB的mRNA水平的表达情况.与对照组相比,甘草多糖能够促进A549细胞活力升高,而LPS处理A549细胞后细胞活力显著下降.ELISA法检测结果表示,相比对照组,LPS处理后细胞炎症因子TNF-α和IL-6的含量显著升高,LPS和甘草多糖复合处理后A549细胞内的TNF-α和IL-6因子显著降低.进一步实时荧光定量PCR结果表明,LPS组中细胞IL-6、IL-1β和NF-κB炎症因子mRNA的表达明显升高,甘草多糖处理后细胞中IL-6、IL-1β和NF-κB炎症因子mRNA的表达显著降低.与LPS组相比,甘草多糖能够显著降低炎症因子mRNA的表达.综上所述,甘草多糖对LPS诱导的A549细胞炎症损伤起拮抗作用,其可能机理是通过抑制NF-κB信号通路.Glycyrrhiza is one of the most widely used herbaceous plants in the world,and its main ex-tract of glycyrrhiza polysaccharide is a polysaccharide compound with various biological activities.Aims to ex-plore the molecular mechanism of the antagonistic effect of glycyrrhiza polysaccharide(GP)on lipopolysaccha-ride(LPS)induced inflammation of A549 cells,and provide a theoretical basis for the treatment of pulmonary in-flammatory diseases.The crude extract of glycyrrhiza polysaccharides was extracted using water extraction and alcohol precipitation method.Using the cell proliferation detection CCK-8 kit to detect the effects of different concentrations of glycyrrhiza polysaccharides and LPS on the viability of A549 cells.The expressions of TNF-a and IL-6 factors in cells was detected by enzyme-linked immunosorbent assay(ELISA).The expression of NF-kB,IL-1βand IL-6 mRNA levels was detected by real-time fluorescence quantitative PCR in A549 cells.The results showed that compared with the control group,glycyrrhiza polysaccharide were promoted an increase in A549 cell viability,while LPS treatment significantly reduced A549 cell viability.The ELISA test results showed that compared to the control group,the content of cell inflammatory factors TNF-a and IL-6 signifi-cantly increased by LPS treated.Further,the content of TNF-a and IL-6 were significantly reduced by LPS and glycyrrhiza polysaccharide and complex treatment in A549 cells.In addition,the real-time fluorescence quantita-tive PCR results showed that the expression of IL-6、IL-1βand NF-kB inflammatory factors were significantly increased in the LPS group,and the mRNA expressions of IL-6、IL-1βand NF-kB inflammatory factors were significantly decreased after treatment with glycyrrhiza polysaccharide.Glycyrrhiza polysaccharide could signifi-cantly reduce the expression of inflammatory factor mRNA,compared with the LPS group.In summary,glycyr rhiza polysaccharide have an antagonistic effect on LPS induced inflammatory damage possibly through inhibit-ing NF-kB s

关 键 词：甘草多糖 脂多糖 A549细胞 炎症损伤 

分 类 号：TS201.4[轻工技术与工程—食品科学]