Dynamic intrauterine crosstalk promotes porcine embryo implantation during early pregnancy  

作　　者：Xupeng Zang Shengchen Gu Wenjing Wang Junsong Shi Jianyu Gan Qun Hu Chen Zhou Yue Ding Yanjuan He Lei Jiang Ting Gu Zheng Xu Sixiu Huang Huaqiang Yang Fanming Meng Zicong Li Gengyuan Cai Linjun Hong Zhenfang Wu 

机构地区：[1]State Key Laboratory of Swine and Poultry Breeding Industry,National Engineering Research Center for Breeding Swine Industry,College of Animal Science,South China Agricultural University,Guangzhou 510642,China [2]Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding,South China Agricultural University,Guangzhou 510642,China [3]Yunfu Subcenter of Guangdong Laboratory for Lingnan Modern Agriculture,Yunfu 527300,China [4]Guangdong Key Laboratory of Animal Breeding and Nutrition,Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China [5]Key Laboratory of South China Modern Biological Seed Industry,Ministry of Agriculture and Rural Affairs,Guangzhou 510520,China

出　　处：《Science China(Life Sciences)》2024年第8期1676-1696,共21页中国科学（生命科学英文版）

基　　金：supported by the National Key Research and Development Program of China(2021YFD1301103);the National Natural Science Foundation of China(31802033);the Guangdong Provincial Key Area Research and Development Program(2022B0202090002);the Guangdong Basic and Applied Basic Research Foundation(2022A1515011131,2023A1515030193);the Guangdong Provincial Promotion Project on Preservation and Utilization of Local Breed of Livestock and Poultry(2018143).

摘　　要：Dynamic crosstalk between the embryo and mother is crucial during implantation.Here,we comprehensively profile the single-cell transcriptome of pig peri-implantation embryos and corresponding maternal endometrium,identifying 4 different lineages in embryos and 13 cell types in the endometrium.Cell-specific gene expression characterizes 4 distinct trophectoderm subpopulations,showing development from undifferentiated trophectoderm to polar and mural trophectoderm.Dynamic expression of genes in different types of endometrial cells illustrates their molecular response to embryos during implantation.Then,we developed a novel tool,ExtraCellTalk,generating an overall dynamic map of maternal-foetal crosstalk using uterine luminal proteins as bridges.Through cross-species comparisons,we identified a conserved RBP4/STRA6 pathway in which embryonic-derived RBP4 could target the STRA6 receptor on stromal cells to regulate the interaction with other endometrial cells.These results provide insight into the maternal-foetal crosstalk during embryo implantation and represent a valuable resource for further studies to improve embryo implantation.

关 键 词：endometrial remodeling ExtraCellTalk IMPLANTATION maternal-foetal crosstalk TROPHOBLAST 

分 类 号：R714.8[医药卫生—妇产科学]