钛表面多酚介导铜离子涂层制备及抗菌抗氧化性能  

Preparation of polyphenol-mediated copper ion coating on titanium surface and antibacterial and antioxidant properties

作　　者：官振菊 谢永林[2] 向守刚 张成栋 李小龙 李兴平 蒲超[4] 张波[4] 罗栩伟[4] 肖东琴[1] Guan Zhenju;Xie Yonglin;Xiang Shougang;Zhang Chengdong;Li Xiaolong;Li Xingping;Pu Chao;Zhang Bo;Luo Xuwei;Xiao Dongqin(Research Institute of Tissue Engineering and Stem Cells,Nanchong Central Hospital·Second Clinical College of North Sichuan Medical College,Nanchong 637000,Sichuan;Department of Stomatology,Nanchong Central Hospital·Second Clinical College of North Sichuan Medical College,Nanchong 637000,Sichuan;Department of Orthopedics,Chengfei Hospital,Chengdu 610031,Sichuan Province,China;Department of Orthopedics,Nanchong Central Hospital·Second Clinical College of North Sichuan Medical College,Nanchong 637000,Sichuan Province,China)

机构地区：[1]川北医学院第二临床学院·南充市中心医院组织工程与干细胞研究所,四川省南充市637000 [2]川北医学院第二临床学院·南充市中心医院口腔科,四川省南充市637000 [3]成飞医院骨科,四川省成都市610031 [4]川北医学院第二临床学院·南充市中心医院骨科,四川省南充市637000

出　　处：《中国组织工程研究》2025年第10期1997-2005,共9页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(82002289),项目负责人:肖东琴;四川省自然科学基金项目(2022NSFSC0685),项目负责人:肖东琴;四川省自然科学基金项目(2022NSFSC0609),项目负责人:罗栩伟;四川省自然科学基金项目(2023NSFSC1740),项目负责人:张成栋;四川省医学科研课题计划项目(Q22061),项目负责人:蒲超;四川省医学科研课题计划项目(Q22034),项目负责人:李兴平;南充市市校合作科研项目(22SXJCQN0002),项目负责人:肖东琴;南充市市校合作科研项目(22SXQT0386),项目负责人:谢永林;南充市市校合作科研项目(22SXQT0310),项目负责人:张波。

摘　　要：背景:钛植入体因比强度高及良好的生物相容性被广泛应用于临床,然而在植入手术过程中,细菌感染及组织受损环境产生大量活性氧物质易导致组织延迟愈合与手术失败,因此开发具有抗菌和抗氧化性能的钛植入体极为重要。目的:鉴于铜离子良好的抗菌性能及多酚类物质优良的抗氧化性能,拟在钛表面制备多酚介导的铜离子涂层,评价其体外抗菌及抗氧化性能。方法:利用碱热法在钛表面制备纳米结构,然后将钛片置于单宁酸及铜离子溶液中,获得多酚介导的铜离子涂层,检测样品的表面形貌与水接触角,利用原子吸收光谱法检测铜离子的携载及释放。将金黄色葡萄球菌分别接种于纯钛片(空白组)、碱热处理钛片(对照组)、多酚介导的铜离子修饰钛片(实验组)表面,观察细菌存活状态。将成骨前体细胞MC3T3-E1分别接种于3组钛片表面,评价材料的抗氧化性能及生物活性。结果与结论:(1)扫描电镜下可见多酚介导的铜离子修饰钛片表面存在棒状纳米结构,并且涂层无裂纹;多酚介导的铜离子修饰钛片表面亲水性接近纯钛片;原子吸收光谱结果显示,多酚介导后钛片表面铜离子的携载量增加了51%,铜离子呈均匀释放。(2)空白组、对照组、实验组钛片的抗菌率为0%,21.65%,93.75%;活死染色与CTC染色显示,空白组钛片表面的活细菌最多,实验组钛片表面的活细菌最少。(3)活死染色与CCK-8检测结果显示,3组钛片均具有良好的细胞相容性,并且实验组钛片更有利于MC3T3-E1细胞增殖;活性氧荧光探针检测显示,相较于其他两组,实验组钛片表面活性氧荧光强度明显减弱;碱性磷酸酶与茜素红S染色结果显示,相较于其他两组,实验组钛片表面MC3T3-E1细胞的成骨分化与细胞外基质矿化能力更强。(4)结果表明,多酚介导的铜离子涂层具有较强的抗菌、抗氧化及促成骨分化性能。BACKGROUND:Titanium implants are widely used in clinical practice because of their high strength and good biocompatibility.However,during implantation,bacterial infection and tissue damage environment produce a large number of reactive oxygen species,which can easily lead to delayed tissue healing and surgical failure.Consequently,the development of titanium implants with antimicrobial and antioxidant properties becomes paramount.OBJECTIVE:Considering the potent antimicrobial attributes of copper ions and the remarkable antioxidant qualities of polyphenols,we proposed the fabrication of polyphenol-mediated copper ion coatings on titanium surfaces.These coatings were subsequently assessed for their in vitro antimicrobial and antioxidant properties.METHODS:Nanostructures were generated on the titanium surface using the alkali thermal method.The titanium was immersed in a solution containing tannic acid and copper ions to achieve polyphenol-mediated copper ion coatings.The surface morphology and water contact angle were detected.The loading and release of copper ions were examined using atomic absorption spectroscopy.Staphylococcus aureus was inoculated on the surface of pure titanium sheet(blank group),alkali heat treated titanium sheet(control group),and polyphenol mediated copper ion modified titanium sheet(experimental group)to observe the bacterial survival status.Osteoblast precursor cells MC3T3-E1 were co-cultivated on the surface of three groups of titanium sheets to assess their antioxidant properties and bioactivity.RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that the polyphenol-mediated copper ion modified titanium sheet had rod-like nanostructures and no cracks on the surface.The surface hydrophilicity of copper ion modified titanium sheet mediated by polyphenol was close to that of pure titanium sheet.Atomic absorption spectrometry results showed a 51%increase in the loading capacity of copper ions after polyphenol mediation,with a uniform release of copper ions.(2)The antibacterial ra

关 键 词：钛 植入体 抗菌 抗氧化 多酚 铜离子 涂层 

分 类 号：R459.9[医药卫生—治疗学] R318.5[医药卫生—临床医学] R782.12