长链非编码RNAC3orf77-2在下咽鳞癌中的表达及其对增殖和侵袭的影响  

Expression of long-stranded non-coding RNA C3orf77-2 in squamous carcinoma of the hypopharynx and its effect on proliferation and invasion

作　　者：路武豪[1] 张鹏[1] 高佩[1] 张艳飞[1] 冯龙[2] Lu Wuhao;Zhang Peng;Gao Pei;Zhang Yanfei;Feng Long(Department of Otolaryngology-Head and Neck Surgery,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China;Department of Pathogenic Organism Biology,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]郑州大学第一附属医院头颈外科,郑州450052 [2]河南中医药大学医学院,郑州450046

出　　处：《中华实验外科杂志》2024年第7期1541-1544,共4页Chinese Journal of Experimental Surgery

基　　金：河南省高校重点科研项目(18B310029)。

摘　　要：目的探讨长链非编码RNA(lncRNA)C3orf77-2对头颈鳞癌细胞增殖和迁移的影响。方法收集2021年9月至2023年9月郑州大学第一附属医院收治并接受手术治疗的30例下咽鳞癌患者的临床样本作为研究对象。选取下咽鳞癌组织和癌旁组织标本, 运用lncRNA芯片技术检测下咽鳞癌组织和配对的癌旁组织标本中lncRNA的表达, 分析差异表达的非编码RNA;实时定量反转录聚合酶链反应(RT-qPCR)验证基因芯片差异基因;收集临床数据, 分析lncRNA C3orf77-2表达与各种临床病理的相关性;下咽鳞癌FaDu细胞随机分为对照组、siNC组、lncRNA C3orf77-2 si-lnc1组、lncRNA C3orf77-2 silnc2组;沉默lncRNA C3orf77-2后, 采用细胞计数试剂盒(CCK-8)和克隆形成实验检测细胞增殖能力, 细胞划痕实验检测细胞迁移能力;组间比较采用最小显著差异法LSD-t检验进行统计分析。结果下咽鳞癌组织中lncRNA C3orf77-2表达量(0.78±0.14)显著高于癌旁组织(0.13±0.10), 差异有统计学意义(t=14.96, P<0.01)。下咽鳞癌组织中lncRNA C3orf77-2的表达与患者肿瘤分化程度(F=4.3, P<0.05)、TNM分期(F=13.4, P<0.05)、淋巴结转移(t=4.07, P<0.05)明显相关;CCK-8结果显示, 各组细胞在24、48、72 h的吸光度值分别为空白对照组(0.360±0.018、0.546±0.047、0.882±0.028), siNC组(0.401±0.018、0.561±0.037、0.825±0.056), lncRNA C3orf77-2 si-lnc1(0.230±0.031、0.416±0.009、0.555±0.048)和lncRNA C3orf77-2 si-lnc2(0.244±0.008、0.367±0.041、0.531±0.022), 差异有统计学意义(F24 h=15.6、F48 h=360.7、F72 h=105.7, P<0.01)。克隆形成实验结果显示, siNC组(89.00±0.58), lncRNA C3orf77-2 si-lnc1(31.67±1.76), lncRNA C3orf77-2 si-lnc2(22.67±1.76), 空白对照组(94.00±2.52), 差异有统计学意义(F=433.6, P<0.01)。细胞划痕实验显示:si-NC组(36.67±4.33), lncRNA C3orf77-2 si-lnc1(10.83±1.17), lncRNA C3orf77-2 si-lnc2(12.00±1.67), 差异有统计学意义(F=78.8, P<0.01)。结论 lncRNA C3orf77-2在下咽鳞�Objective To investigate the effect of long chain non-coding RNA(lncRNA)C3orf77-2 on the proliferation and migration of head and neck squamous carcinoma cells.Methods Clinical samples from 30 patients with squamous carcinoma of the hypopharynx admitted to the First Afiliated Hospital of Zhengzhou University and underwent surgical treatment from September 2021 to September 2023 were collected as study subjects.Hypopharyngeal squamous carcinoma tissues and paracancerous tissue speci-mens were selected,and IncRNA microarray technology was used to detect the expression of long-chain noncoding RNAs in hypopharyngeal squamous carcinoma tissues and paired paracancerous tissue speci-mens,and to analyze the differentially expressed noncoding RNAs;RT-qPCR was performed to validate the differential genes of gene microarray;and the clinical data were collected and analyzed for the correlation between the expression of lncRNA C3orf77-2 and various clinicopathologies;Hypopharyngeal squamous carcinoma FaDu cells were randomly divided into control group,siNC group,lncRNA C3orf77-2 si-lncl group,lncRNA C3orf77-2 silnc2 group;after silencing of lncRNA C3orf77-2,cell proliferation ability was detected by cell counting kit-8(CCK-8)and clone formation assay,and cell migration ability was detected by cell scratch assay;the comparison between groups was made by the least significant difference method LSD;the comparison between groups was made by the least significant difference method LSD.Comparisons between groups were statistically analyzed using the least significant difference method LSD-t test.Results The expression of IncRNA C3orf77-2 in hypopharyngeal squamous carcinoma tissues(0.78±0.14)was significantly higher than that in paracancerous tissues(0.13±0.10),and the difference was statistically significant(t=14.96,P<0.01).The expression of IncRNA C3orf77-2 in hypopharyngeal squamous carcinoma tissues was significantly correlated with the degree of tumor differentiation(F=4.3,P<0.05),TNM stage(F=13.4,P<0.05),and lymph node metas

关 键 词：长链非编码RNA 下咽鳞癌 增殖 迁移 

分 类 号：R739.63[医药卫生—肿瘤]