长链非编码RNA浆细胞瘤变异易位1在胃癌中的表达及临床意义  

Expression and clinical significance of long noncoding RNA plasmacytoma variant translocation 1 in gastric

作　　者：陈文亮[1] 董静逊[2] 白子豪 王欢欢 郝金锦 陈凛 Chen Wenliang;Dong Jingxun;Bai Zihao;Wang Huanhuan;Hao Jinjin;Chen Lin(Department of General Surgery,the 2nd Affiliated Hospital of Shanxi Medical University,Taiyuan 030001,China;Department of Minimal Invasive Digestive Surgery,Tumor Hospital of Shanxi Medical University,Taiyuan 030013,China;Graduate Department of Shanxi Medical University,Taiyuan 030001,China;Department of Gastrointestinal Surgery,Peking University International Hospital,Beijing 102206,China)

机构地区：[1]山西医科大学第二医院普通外科,太原030001 [2]山西省肿瘤医院消化微创外科,太原030013 [3]山西医科大学研究生学院,太原030001 [4]北京大学国际医院胃肠外科,北京10220

出　　处：《中华实验外科杂志》2024年第7期1554-1557,共4页Chinese Journal of Experimental Surgery

基　　金：山西省自然科学基金面上项目(201801D121320)。

摘　　要：目的探讨长链非编码RNA PVT1(lncRNA PVT1)在胃癌(GC)组织中的表达及临床意义。方法首先通过实时荧光定量聚合酶链反应(RT-qPCR)检测196例GC组织中lncRNA PVT1表达, 采用χ^(2)检验分析其与临床病理特征间关系。利用t检验分析26例GC组织中微小RNA(miR)-30a-3p表达;然后对具有随访资料的182例GC患者, 进行生存分析和Cox回归分析。结果 RT-qPCR检测结果显示:癌组织中lncRNA PVT1的表达水平显著高于癌旁组织(0.33±0.15比0.28±0.13, t=3.746, P<0.01)。lncRNA PVT1表达水平与GC肿瘤大小(χ^(2)=8.610, P<0.01)、癌组织分化程度(χ^(2)=4.927, P<0.05)、浸润深度(χ^(2)=5.940, P<0.05)、pTNM分期(χ^(2)=4.675, P<0.05)及淋巴结转移(χ^(2)=4.712, P<0.05)呈现显著相关性。而GC组织中miR-30a-3p的表达水平显著低于癌旁组织(0.210±0.156比0.302±0.177, t=2.378, P<0.05)。生存分析显示:lncRNA PVT1高表达组患者总生存期(OS)明显短于低表达组[风险比(HR)=1.746, 95%可信区间(CI):1.154~2.642, P<0.01]。结论 lncRNA PVT1在GC组织中存在表达上调, miR-30a-3p在GC组织中表达下调, lncRNA PVT1的表达水平与GC患者的临床病理特征明显相关, 并且与GC患者的预后明显相关。Objective To explore the expression and clinical significance of long noncoding RNA PVT1(lncRNA PVT1)in gastric cancer(GC)tissues.Methods Real-time quantitative fluorescent PCR(RT-qPCR)was used to detect lncRNA PVT1 expression in 196 GC tissues,and the relationship between IncRNA PVT1 expression and clinicopathological features was analyzed by 2 assay.MicroRNA(miRNA,miR)-30a-3p expression in 26 GC tissues was analyzed by t test.Survival analysis was performed by Kaplan-Merier method in 182 GC patients with follow-up data.Results The expression level of lncRNA PVT1 in cancer tissues was significantly higher than that in para-cancer tissues(0.33±0.15 vs.0.28±0.13,t=3.746,P<0.01).IncRNA PVT1 expression level was corelated with CC tumor size(χ^(2)=8.610,P<0.01),the degree of differentiation of cancer tissue(χ^(2)=4.927,P<0.05),infiltration depth(χ^(2)=5.940,P<0.05),pTNM staging(χ^(2)=4.675,P<0.05)and lymph node metastasis(χ^(2)=4.712,P<0.05).The expression level of miR-30a-3p in GC tissues was significantly decreased as compared with that in para-cancer tissues(0.210±0.156 vs.0.302±0.177,t=2.378,P<0.05).Survival analysis showed that the overall survival(OS)of patients with high lncRNA PVT1 expression was significantly shor-ter than that of patients with low expression[hazard ratio(HR)=1.746,95%confidence interval(CI):1.154-2.642,P<0.01].Conclusion The expression of lncRNA PVT1 is up-regulated in GC tissues,and the expression of miR-30a-3p is down-regulated in GC tissues.The expression level of IncRNA PVT1 is significantly correlated with the clinicopathological features and the prognosis of GC patients.

关 键 词：胃癌 微小RNA 预后 

分 类 号：R735.2[医药卫生—肿瘤]