核糖体组装调控因子在结直肠癌组织的表达及其临床意义  

Expression and clinical significance of ribosome assembly regulatory factors in colorectal cancer tissue

作　　者：程骏驰 王小红[1] Cheng Junchi;Wang Xiaohong(Department of Colorectal Surgery,Zhejiang Cancer Hospital,Hangzhou 310005,China)

机构地区：[1]浙江省肿瘤医院结直肠科,杭州310005

出　　处：《中华实验外科杂志》2024年第7期1567-1570,共4页Chinese Journal of Experimental Surgery

基　　金：浙江省中医药管理局(2015ZA035)。

摘　　要：目的通过生物信息学的方法探究核糖体组装调控因子(PNO1)在结直肠癌(CRC)中的表达及和临床病理、预后的相关性。方法在癌症基因组图谱(TCGA)数据库内将结直肠转录组测序技术(RNA-seq)相关数据下载, 包含了43例正常结直肠样本和475例结直肠癌组织样本。选取PNO1在结直肠癌内表达的中位数值, 把结直肠癌患者分为表达高水平与低水平组, 探究患者病理资料和PNO1表达的关联度。对高表达和低表达组患者生存率差异应用Kaplan-Meier法分析。PNO1表达对患者预后的影响应用Cox回归分析。京都基因和基因组(KEGG)通路分析PNO1在CRC内所参加的相关通路。结果结直肠癌组织内PNO1 mRNA表达水平显著高于正常组织(χ^(2)=12.312, P<0.05)。在TCGA数据集内, 通过对43对配对样本比较显示, 结直肠癌组织内PNO1表达水平显著高于正常组织(χ^(2)=16.503, P<0.05)。PNO1表达水平在患者年龄、发病部位、性别、神经侵犯、脉管侵犯、TNM分期和肿瘤大小方面比较差异无统计学意义(χ^(2)=1.730、0.307、0.990、0.990、0.848、0.545、1.806, P>0.05);PNO1表达水平在患者淋巴结转移、浸润深度和分化程度方面比较差异有统计学意义(χ^(2)=6.275、47.943、11.479, P<0.05)。两组患者总体生存率采用Kaplan-Meier法分析显示, 与PNO1低表达组比较, 高表达组患者总体生存率显著降低(χ^(2)=11.627P<0.05)。OncoLnc数据库显示PNO1 mRNA高表达组患者的生存率低于低表达组(χ^(2)=17.105P<0.05)。单因素Cox回归分析表明, 影响患者预后因素有PNO1表达、年龄、分化程度、淋巴结转移、浸润程度和临床分期, 差异有统计学意义[风险比(HR)=1.284、1.034、1.162、2.021、1.314、2.843;95%可信区间(CI)=1.101~1.533、1.010~1.055、1.084~1.530、1.556~2.680、1.124~1.628、1.814~4.356, P<0.05];多因素Cox回归分析表明, 影响患者预后的独立因素为PNO1表达、临床分期和年龄(HR=1.290、1.743、1.Objective To explore the expression of ribosomal assembly regulatory factor(PNO1)in colorectal cancer(CRC)and its correlation with clinicopathological features and prognosis through bioinformatics methods.Methods Download colorectal transcriptome sequencing technology(RNA seq)related data from the Cancer Genome Atlas(TCGA)database,including 43 normal colorectal samples and 475 colorectal cancer tissue samples.Select the median value of PNO1 expression in colorectal cancer,divide the colorectal cancer patients into high-level and low-level expression groups,and explore the correlation between patient pathological data and PNO1 expression.Apply the Kaplan-Meier method to analyze the difference in survival rate between high-expression and low-expression groups of patients.Apply Cox regression analysis to investigate the impact of PNO1 expression on patient prognosis.Analyze the relevant pathways in which PNOl participates in CRC using kyoto encyclopedia of genes and genomes(KEGG)pathway analysis.Results The expression level of PNOl mRNA in colorectal cancer tissue is significantly higher than that in normal tissue(χ^(2)=12.312 P<0.05).In the TCGA dataset,a comparison of 43 paired samples showed that the expression level of PNO1 in colorectal cancer tissue was significantly higher than that in normal tissue(χ^(2)=16.503 P<0.05).There is no statistically significant difference in PNO1 expression levels among patients in terms of age,site of onset,gender,nerve invasion,vascular invasion,TNM stag-ing,and tumor size(χ^(2)=1.730,0.307,0.990,0.990,0.848,0.545,1.806,P>0.05);There is a statistically significant difference in the expression level of PNO1 in patients with lymph node metastasis,infiltration depth,and differentiation degree(χ^(2)=6.275,47.943,11.479,P<0.05).The Kaplan Meier method was used to analyze the overall survival rate of two groups of patients,and it was found that compared with the low expression group of PNOl,the high expression group had a significant decrease in overall survival rate(χ^(2)=11.62

关 键 词：生物信息学 结直肠癌 核糖体组装调控因子 病理特征 

分 类 号：R735.34[医药卫生—肿瘤]