Circ_0001946靶向miR-671-5p调控Wnt/β-catenin通路对结直肠癌细胞恶性进展的影响  

Circ_0001946 Promotes Malignant Progression of Colorectal Cancer Cells by Activating Wnt/β-catenin Pathway Through miR-671-5p

作　　者：邹燕[1] 刘立民[1] 蒙杰[1] 巢薇[1] 王国栋[1] 李远眺[1] ZOU Yan;LIU Limin;MENG Jie;CHAO Wei;WANG Guodong;LI Yuantiao(Liuzhou Worker's Hospital,Liuzhou 545005,China)

机构地区：[1]柳州市工人医院,广西柳州545005

出　　处：《肿瘤学杂志》2024年第6期458-466,共9页Journal of Chinese Oncology

基　　金：广西壮族自治区卫生和计划生育委员会自筹经费科研课题(Z20190002,Z20190296,Z20200472);广西自然科学基金青年基金项目(2019GXNSFBA185003);柳州市科学研究与技术开发计划课题(2017BH20306);广西高校中青年教师科研基础能力提升项目(2019KY0156)。

摘　　要：[目的]探讨circ_0001946对结直肠癌细胞恶性进展的影响。[方法]培养结直肠癌HCT116、SW480、Colo320、HT29细胞和正常结直肠CCC-HIE-2细胞,实时定量PCR检测circ_0001946、miR-671-5p表达量。miRcode和双荧光素酶报告实验分析circ_0001946和miR-671-5p的关系。circ_0001946、miR-671-5p在HCT116细胞中的表达量被上调或下调后,分别检测HCT116细胞生长活性、细胞凋亡率及细胞迁移能力。免疫蛋白印迹法检测Bcl-2、Bax、Caspase-3和Caspase-9蛋白表达及Wnt/β-catenin信号通路蛋白表达。[结果] HCT116 (4.76±0.47)、HT29 (2.85±0.57)、SW480 (2.75±0.62)、Colo320 (3.12±0.68)细胞中circ_0001946表达水平高于CCC-HIE-2细胞(1.24±0.09)(t=7.238、8.129、6.893、6.875,P均<0.05)。下调circ_0001946表达显著降低了HCT116细胞增殖(P<0.05)和迁移能力(P<0.05),促进了细胞凋亡(P<0.01);circ_0001946靶向miR-671-5p;circ_0001946表达被上调或miR-671-5p表达被下调后,HCT116细胞增殖和迁移能力显著上升,细胞凋亡率明显降低,Wnt/β-catenin信号通路被激活。[结论] circ_0001946靶向miR-671-5p激活了Wnt/β-catenin信号通路,促进了结直肠癌细胞恶性进展。[Objective]To investigate the effect of circ_0001946 on the malignant progression of colorectal can-cer cells and its mechanism.[Methods]The expression levels of circ_0001946 and miR-671-5p were detected in human colorectal carcinoma HCT116,SW480,Colo320,HT29 cells and normal colorectal CCC-HIE-2 cells by RT-qPCR.The relationship between circ_0001946 and miR-671-5p was analyzed by miRcode and du-al luciferase reporting assay.After circ_0001946 and miR-671-5p expression in HCT116 cells were up-regulat-ed or down-regulated,the growth activity,apoptosis rate and migration ability of HCT116 cells were detected respectively.The expression of Bcl-2,Bax,Caspase-3 and Caspase-9 and the proteins of Wnt/β-catenin sig-naling pathway were detected by Western blot.[Results]The expression levels of circ_0001946 in HCT116(4.76±0.47),HT29(2.85±0.57),SW480(2.75±0.62),and Colo320(3.12±0.68)cells were significantly higher than those in CCC-HIE-2 cells(1.24±0.09)(t=7.238,8.129,6.893,6.875,all P<0.05).Down-regulation of circ_0001946 significantly reduced the proliferation(P<0.05)and migration(P<0.05)of HCT116 cells,and promoted cell apoptosis(P<0.01).The dual luciferase reporting assay showed that circ_0001946 targeted miR-671-5p.Up-regulation of circ_0001946 or down-regulation of miR-671-5p significantly enhanced proliferation and migration of HCT116 cells,decreased apoptosis rate,and activated Wnt/β-catenin signaling pathway.[Conclusion]The study indicates that circ_0001946 may promote malignant progression of colorectal cancer cells by activating the Wnt/β-catenin signaling pathway through miR-671-5p.

关 键 词：结直肠癌细胞 恶性进展 miR-671-5p CATENIN WNT 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]