拟微绿球藻发酵肽的抗衰老作用研究  

The anti-aging potential of peptides from fermented Nannochloropsis sp.

作　　者：杨婷 李华文 许训 郭红辉 邹堂斌 夏恩琴 Ting Yang;Huawen Li;Xun Xu;Honghui Guo;Tangbin Zou;Enqin Xia(Department of Nutrition,School of Public Health,Guangdong Medical University,Dongguan,Guangdong 523808,China;Dongguan Key Laboratory for Development and Application of Experimental Animal Resources in Biomedical Industry,Guangdong Medical University,Dongguan,Guangdong 523808,China;Laboratory Animal Center,Guangdong Medical University,Dongguan,Guangdong 523808,China;Shunde Women and Children’s Hospital,Guangdong Medical University,Guangzhou,Guangdong 528399,China)

机构地区：[1]广东医科大学公共卫生学院,广东东莞523808 [2]东莞市生物医药产业实验动物资源开发应用重点实验室,广东东莞523808 [3]广东医科大学实验动物中心,广东东莞523808 [4]广东医科大学附属顺德妇幼保健院,广东广州528399

出　　处：《日用化学工业（中英文）》2024年第8期921-929,共9页China Surfactant Detergent & Cosmetics

基　　金：广东省基础与应用基础研究基金联合基金项目(2021B1515140057);广东省医学科研基金项目(A2023434)。

摘　　要：探讨了拟微绿球藻发酵肽(FNPs)的提取、组成分析及其对果蝇体内抗衰老效果。与非发酵组相比,FNPs的多肽含量增加约100条,肽中的抗氧化位点显著增加。FNPs的最大吸收强度在258 nm处,且具有剂量依赖关系。在果蝇实验中,经口干预后,5μg/mL FNPs可使自然衰老的黑腹果蝇的平均寿命延长约13天。同时观察到,在70~90日龄时,0.5,5和10μg/mL的FNPs均可缓解因年龄引起的MDA、SOD和CAT等氧化应激指标的恶化,并将其提高到阳性组水平。结果表明,FNPs是潜在的缓解自然衰老引起的健康问题的生物活性成分,其资源丰富且易于获得,在作为新型抗氧化成分进行功能性食品开发或营养保健膳食制剂将有很好的应用前景。The extraction and composition analysis of peptides(FNPs)from fermentation Nannochloropsis sp.,as well as their anti-aging effects on Drosophila melanogaster in vivo were investigated.Compared with the non-fermentation group,the number of peptide in FNPs increases by about 100,and the oxidation sites in the peptides also increase.The maximum absorption intensity of FNPs is observed at 258 nm with a dose-dependent relationship.Compared with the control group,5μg/mL FNPs extends the mean life span of the the natural aging Drosophila melanogaster for 13 days.Additionally,for 70-90 days old,the deterioration of oxidative stress indicators such as MDA,SOD,and CAT caused by age are significantly restored and increased to the level of positive group by FNPs at 0.5 and 5μg/mL.These results indicate that FNPs have potential as a promising nutritional component for alleviating human health problems induced by aging.It can also be used as a new antioxidant ingredient for functional food development or cosmeceutical applications from a resource rich and easily obtainable raw material Nannochloropsis sp..

关 键 词：生物活性肽 抗衰老 拟微滤球藻发酵肽 黑腹果蝇 

分 类 号：TQ658[化学工程—精细化工]