基于双菌偶联分阶段培养诱导酵母细胞壁释放甘露聚糖的研究  

Study on induction of yeast cell wall release of mannan through phase wise culture of two bacteria coupling

作　　者：贡璐茜 张高瑜 王虹[1] 王东旭 吕雪盟 王思佳 刘金龙[1,2] GONG Lu-qian;ZHANG Gao-yu;WANG Hong;WANG Dong-xu;LÜXue-meng;WANG Si-jia;LIU Jin-long

机构地区：[1]河北科技大学食品与生物学院,河北石家庄050018 [2]河北省发酵技术创新中心,河北石家庄050018

出　　处：《饲料研究》2024年第13期70-74,共5页Feed Research

基　　金：河北省自然科学基金(项目编号:C2021208019)。

摘　　要：试验旨在实现酵母细胞壁甘露聚糖的高效释放及寡糖化。试验提出一种新型的双菌偶联分阶段培养模式,诱导酵母细胞壁释放甘露聚糖,通过筛选诱导菌株、双菌分阶段发酵验证释放多糖的效果。结果显示,试验通过对比测定4种芽孢杆菌对酿酒酵母细胞壁破壁率、甘露聚糖释放率的影响,确立了枯草芽孢杆菌-FMTK01-酿酒酵母双菌分阶段培养模式。双菌分阶段培养72 h时,酵母破壁率达到96.63%,甘露聚糖释放量达到83.87%。经过甘露聚糖解聚化分析可知,双菌分阶段培养模式实现了37.27%的酵母甘露聚糖的寡糖化。研究表明,试验提出的双菌偶联分阶段培养发酵模式可加速酵母细胞壁释放甘露聚糖以及多糖寡糖化,对其他生物多糖及其寡糖的制备具有一定的借鉴意义。The experiment aimed to achieve the efficient release and oligosaccharidation of mannan from yeast cell walls.A novel dual-strain coupled phased cultivation model is proposed in the experiment to induce the release of mannan from yeast cell walls,and the effectiveness of polysaccharide release was verified by screening inducing strains and dual-strain phased fermentation.The results showed that by comparing the effects of four Bacillus strains on the cell wall rupture rate and mannan release rate of brewer's yeast,a Bacillus subtilis-FMT-K01-Saccharomyces cerevisiae dual-strain phased cultivation model was established.After 72 hours of dual-strain phased cultivation,the yeast cell wall rupture rate reached 96.63%,and the mannan release rate reached 83.87%.Through the depolymerization analysis of mannan,it was found that the dual-strain phased cultivation model achieved 37.27%oligosaccharidation of yeast mannan.The study indicates that the dual-strain coupled phased fermentation model proposed in this research can accelerate the release of mannan from yeast cell walls and the oligosaccharidation of polysaccharides,and it has certain reference significance for the preparation of other biological polysaccharides and their oligosaccharides.

关 键 词：酿酒酵母 枯草芽孢杆菌 酵母甘露聚糖 分阶段培养 

分 类 号：S816.7[农业科学—饲料科学]