不同分期牙周炎与人类白细胞抗原DP基因遗传变异的相关性分析  

作　　者：张媛媛[1] 丁成[1] 

机构地区：[1]杭州师范大学附属医院/杭州师范大学口腔医学院,310000

出　　处：《浙江临床医学》2024年第7期1002-1004,共3页Zhejiang Clinical Medical Journal

基　　金：杭州市科技计划项目(20211231Y028)。

摘　　要：目的 探讨不同分期牙周炎与HLA-DP基因遗传变异的相关性。方法 选取牙周炎患者159例,健康对照者169例。用Taqman PCR方法检测研究对象HLA-DP基因位点分型,比较牙周炎与HLA-DP基因遗传变异的相关性。结果 HLA-DP基因rs3077和rs9277535位点基因型在汉族人群中的分布均符合Hardy-Weinberg遗传平衡定律(P>0.05)。结果发现rs9277535位点GG基因型在健康对照组中分布明显高于牙周炎组(χ^(2)=7.56,P=0.02)。多因素Logistic回归分析结果显示:rs9277535位点杂合基因型GA和突变基因型AA基因型均可增加牙周炎的患病风险(P<0.05)。单倍型分析显示,牙周炎患者中AA单倍型分布比例高于健康对照组(OR=0.52,95%CI:0.36~0.78)。rs3077和rs9277535位点多态性在不同分期牙周炎患者中的分布差异有统计学意义(P<0.05)。结论 HLA-DP基因遗传变异在牙周炎的发病风险存在关联,可能影响其发病进程。Objective To explore the correlation between different stages of Periodontal disease and genetic variation of HLA-DP gene.Methods A Case-control study was conducted to collect 159 patients with Periodontal disease and 169 healthy controls.Taqman PCR was used to detect the HLA-DP gene locus typing of subjects,and to compare the correlation between Periodontal disease and HLA-DP gene genetic variation.Results The distribution of HLA-DP gene rs3077 and rs9277535 genotypes in the Han population was consistent with Hardy Weinberg's law of genetic balance(P>0.05).The distribution of rs9277535 GG genotype in the healthy control group was significantly higher than that in the Periodontal disease group(χ^(2)=7.56,P=0.02).Multivariate Logistic regression analysis showed that both the heterozygous genotype GA and the mutant genotype AA at rs9277535 locus increased the risk of Periodontal disease(P<0.05).The Haplotype analysis showed that the distribution proportion of AA Haplotype in Periodontal disease patients was significantly higher than that in the healthy control group(OR=0.52,95%CI:0.36~0.78).The results showed that the distribution of rs3077 and rs9277535 loci polymorphism in patients with Periodontal disease in different stages was statistically different(P<0.05).Conclusion Genetic variation of HLA-DP gene is associated with the risk of Periodontal disease and may affect its pathogenesis.

关 键 词：牙周炎 分期 单核苷酸多态性 HLA-DP 

分 类 号：R781.42[医药卫生—口腔医学]