NaClO和KMnO_(4)对沼蛤毒理效应的代谢组学研究  

Metabolomics Study on Toxicological Effect of NaClO and KMnO_(4) on Intrusive Limnoperna fortunei

作　　者：蒲淑娟 袁海光 张锐坚 未碧贵[1] 王堑傧 董浩韬 刘颖诗 PU Shujuan;YUAN Haiguang;ZHANG Ruijian;WEI Bigui;WANG Qianbin;DONG Haotao;LIU Yingshi(School of Environmental and Municipal Engineering,Lanzhou Jiaotong University,Lanzhou 730070,China;Guangdong Yuehai Pearl River Delta Water Supply Co.,Ltd.,Guangzhou 511455,China;Research Center,Guangzhou Municipal Engineering Design&Research Institute,Guangzhou 510060,China)

机构地区：[1]兰州交通大学环境与市政工程学院,甘肃兰州730070 [2]广东粤海珠三角供水有限公司,广东广州511455 [3]广州市市政工程设计研究总院有限公司,广东广州510060

出　　处：《环境科学与技术》2024年第6期1-11,共11页Environmental Science & Technology

基　　金：国家自然科学基金项目(52060014);广东省水利厅水利科技创新项目(2023-03);广东省基础与应用基础研究基金项目(2021A1515010499)。

摘　　要：淡水贻贝沼蛤入侵输水工程形成生物污损影响结构安全,常用的化学防治手段投加浓度仍需深入探究,因此分析NaClO、KMnO_(4)等常用药剂对沼蛤的毒理效应尤为重要。该研究通过沼蛤的死亡率、酶活性和过氧化脂质分别对NaClO和KMnO_(4)的应激响应,采用综合生物标志物响应(IBR)评价沼蛤受到的毒理效应,运用LC-MS非靶向代谢组学分别分析沼蛤鳃组织暴露在NaClO和KMnO_(4)溶液中72 h后的代谢变化,从分子生物学角度揭示沼蛤对NaClO和KMnO_(4)的毒理代谢调节机制,为确定准确的投加浓度提供依据。结果表明:(1)随NaClO浓度的增加,沼蛤鳃组织的抗氧化酶(超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx))活性下降;当暴露在KMnO_(4)溶液中时,抗氧化酶活性先升后降,最大值出现在2.0 mg/L,然后随着浓度的增加抗氧化酶活性下降;表征组织损伤的过氧化脂质(LPO)水平随药剂浓度的增加而增加;(2)死亡率和IBR结果表明,浓度小于4.0 mg/L时,NaClO灭活效果优于KMnO_(4),而当浓度为8.0 mg/L时,KMnO_(4)则优于NaClO;(3)代谢组学分析显示,所有处理组中均发现了氨基酸代谢通路,仅在KMnO_(4)浓度为8.0 mg/L时,处理组中未发现嘌呤代谢以及醚脂代谢通路;浓度小于4.0 mg/L时,NaClO通路以醚脂代谢主,当大于4.0 mg/L时,通路以嘌呤代谢为主;当浓度为8.0 mg/L时,KMnO_(4)通路以氨基酸代谢为主。研究结果可为NaClO和KMnO_(4)防治长距离输水系统的沼蛤生物污损提供微观基础数据和浓度投加的确定依据。The intrusion of freshwater mussel Limnoperna fortunei into water conveyance projects could cause bio-fouling that affects structural safety,and the commonly used chemicals for fouling control still need to be further investigated,so the analysis of the mussels to the toxicological effects of fouling-control agents,such as NaClO and KMnO_(4),is particularly important.In this paper,through analyzing the mussels’responses to the stress by NaClO and KMnO_(4) in terms of enzyme activity and lipid peroxide,the integrated biomarker response(IBR),were used to evaluate the toxicological effects of NaClO and KMnO_(4) on L.fortunei.In addition,LC-MS non-targeted metabolomics was used to analyze the metabolic changes in the gill tissues of the mussels after being exposed to NaClO and KMnO_(4) for 72 hours in order to investigate the toxicological and metabolic regulation mechanisms of NaClO and KMnO_(4) from a molecular biology point of view,thus laying a basis for accurately determining an effective dosage.The results of the study are briefly concluded as follows:(1)The activities of antioxidant enzymes(superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GPx))in the gill tissues decreased with an increase of NaClO concentration;the activities of the antioxidant enzymes first increased,then decreased when mussels were exposed to KMnO_(4) in solution,i.e.,the maximum activity appeared when KMnO_(4) concentration being 2.0 mg/L,but decreased as the increase of the KMnO_(4) concentration;and lipid peroxide level which was used to characterize the tissue damage,went up with the increase of the agents concentration;(2)The results of evaluation using IBR,and the analysis of the mortality suggested that with regard to the in-activation effectiveness,NaClO was better than KMnO_(4) as NaClO concentrations<4.0 mg/L,while KMnO_(4) was better than NaClO as KMnO_(4) concentration being 8.0 mg/L;and(3)Metabolomics analysis showed that the amino-acid metabolism pathways were present in all of the treatment groups,and only

关 键 词：NACLO KMnO_(4) 沼蛤 毒理效应 综合生物标志物响应 代谢组学 

分 类 号：X171.5[环境科学与工程—环境科学]