基于Apt@Au传感器的微囊藻毒素-LR传感检测  

Sensing Detection of Microcystin-LR with Apt@Au Sensor

作　　者：胥均艳 何薪宇 徐溢[1,2,3] XU Junyan;HE Xinyu;XU Yi(Key Disciplines Lab of Novel Micro-nano Devices and System Technology&Key Lab of Optoelectronic Technology and Systems,Ministry of Education,Chongqing University,Chongqing 400044,China;School of Chemistry and Chemical Engineering,Chongqing University,Chongqing 400044,China;School of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China)

机构地区：[1]重庆大学新型微纳器件与系统技术重点学科实验室&光电技术与系统教育部重点实验室,重庆400044 [2]重庆大学化学化工学院,重庆400044 [3]重庆大学光电工程学院,重庆400044

出　　处：《环境科学与技术》2024年第6期105-112,共8页Environmental Science & Technology

基　　金：国家自然科学基金项目(62071072);国家重点研发计划项目(2020YFB2009001);智能传感与微纳生化系统项目(2019研究生导师团队)。

摘　　要：微囊藻毒素(MC-LR)是国际公共卫生组织已确认的一种神经毒素和致癌物质,其高效检测备受关注。该文通过金硫键作用将适配体直接结合到丝网印刷金电极表面,形成适配体@金(Apt@Au)敏感膜,当样本中存在MC-LR时,敏感膜上的适配体与MC-LR相互作用,引起[Fe(CN)_(6)]^(3-)/[Fe(CN)_(6)]^(4-)探针电对的电子传导发生变化,差分脉冲伏安(DPV)响应电流增加,由此构建了一种用于MC-LR检测的Apt@Au传感器。在优化的实验条件下,采用该传感器检测MC-LR的线性范围为0.001~50.00μg/L,检出限可达0.001μg/L,对微囊藻毒素-LA、微囊藻毒素-YR、节球藻毒素以及水体中常见的金属离子无明显响应,表现出良好的选择性。将其用于合成水样检测也显示出良好的回收率。Apt@Au传感器用于MC-LR检测具有流程简单、检测灵敏度高等优势,拥有良好的应用前景。Much attention has been given to the efficient detection of Microcystin-LR(MC-LR),a kind of neurotoxin and car⁃cinogen that has been verified by WHO.This paper describes the trial-producing of a sensor for MC-LR detection,which involves thiol aptamers being directly bonded to the gold electrode surface of the screen-printed electrode by the action of gold sulfur bonds,forming an Apt@Au sensitive interface.The aptamer on the sensitive interface interacts with MC-LR in the sample,causing changes in electron conduction of[Fe(CN)_(6)]^(3-)/[Fe(CN)_(6)]^(4-)and an increase in differential pulse voltammetry(DPV)response current,thus an Apt@Au sensor for MC-LR detection is made.Under the optimized testing condition,the sensor’s detection lin⁃ear range is between 0.001~50.00μg/L,and the detection limit reaches 0.001μg/L.Being good in selectivity,the sensor shows no significant signal response to Microcystin-LA,Microcystin-YR,nodularin toxin,and metal ions commonly found in the water body,and its application to synthetic water samples shows good recovery rates.In short,the Apt@Au sensor for detecting MC-LR has advantages in terms of simple operation flow and high detection sensitivity,showing a good application prospect.

关 键 词：MC-LR Apt@Au传感器 DPV检测 环境水样 

分 类 号：X132[环境科学与工程—环境科学]