磁性多壁碳纳米管固定化脂肪酶及其催化合成1,3-二油酰-2-棕榈酰甘油的研究  

Study on immobilized lipase of magnetic multi-carbon nanotubes and its catalytic synthesis of OPO

作　　者：廖长保 王亚光 彭越 刘娜 吴敬[1] 于殿宇[3] Liao Changbao;Wang Yaguang;Peng Yue;Liu Na;Wu Jing;Yu Dianyu(College of Food Science and Technology,Inner Mongolia Agricultural University,Hohhot 361003;Heilongjiang Hongxing Group Food Co.,Ltd.,Mudanjiang 150070;College of Food Science,Northeast Agricultural University,Harbin 150030)

机构地区：[1]内蒙古农业大学食品科学与工程学院,呼和浩特361003 [2]黑龙江红星集团食品有限公司,牡丹江150070 [3]东北农业大学食品学院,哈尔滨150030

出　　处：《粮食与食品工业》2024年第4期39-44,共6页Cereal & Food Industry

基　　金：黑龙江省重点研发项目“含OPO母乳化脂肪酸高端有机婴幼儿配方奶粉研发”(GA22B017)。

摘　　要：本试验采用磁性多壁碳纳米管固定化疏棉状嗜热丝孢菌脂肪酶(TLL)用于制备1,3-二油酰-2-棕榈酰甘油(OPO)。研究了TLL添加量、反应时间、反应温度对固定化条件的影响以及固定化TLL添加量、反应时间、反应温度和底物摩尔比对制备OPO的影响。结果表明:固定化酶具有更好的耐酸碱性和热稳定性,固定化最优反应条件为酶添加量6%,反应时间5 h,反应温度45℃;合成OPO的最佳条件为反应时间为6 h,反应温度为50℃,酶添加量为8%,底物摩尔比为5∶1。In this paper,magnetic multi-walled carbon nanotubes were used to immobilize the lipase of Thermoanaerobacter sparsus thermophilus(TLL)for the preparation of 1,3-dioleoyl-2-palmitoylglycerol(OPO).The effects of TLL addition,reaction time,and reaction temperature on the immobilization conditions as well as the effects of immobilized TLL addition,reaction time,reaction temperature,and substrate molar ratio on the preparation of OPO were investigated.The results showed that the immobilised enzyme had better acid and alkali resistance and thermal stability.The optimal reaction conditions for the immobilisation were 6%enzyme addition,5 h reaction time and 45℃;the optimal conditions for the synthesis of OPO were 6 h reaction time,50℃,8%enzyme addition and 5∶1 substrate molar ratio.

关 键 词：磁性多壁碳纳米管 固定化 脂肪酶 1 3-二油酰-2-棕榈酰甘油 

分 类 号：TS224[轻工技术与工程—粮食、油脂及植物蛋白工程]