牛马藤多糖的提取工艺优化及抗氧化活性研究  

Optimization of Extraction Process and Antioxidant Activity of Polysaccharides from Mucuna sempervirens

作　　者：邓莹[1] 刘杰[1] 李海洋[1] 陈莎 刘继雄 李明霞 龙昭军 李小丹 DENG Ying;LIU Jie;LI Hai-yang;CHEN Sha;LIU Ji-xiong;LI Ming-xia;LONG Zhao-jun;LI Xiao-dan(Qiannan Medical College for Nationalities,Guizhou Duyun 558000,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558000

出　　处：《广州化工》2024年第11期28-32,共5页GuangZhou Chemical Industry

基　　金：黔南民族医学高等专科学校科研基金项目(Qnyz2021010);黔南州科技计划项目(黔南科合[2022]22号)。

摘　　要：为明确牛马藤多糖的提取工艺条件及其抗氧化活性,为牛马藤多糖的开发利用提供参考。采用了苯酚-硫酸法测定牛马藤多糖的含量,通过单因素试验(以提取温度、液固比、提取时间、提取次数为参考因素),正交试验对牛马藤多糖的提取工艺进行优化,DPPH法和ABTS法测定牛马藤多糖的抗氧化活性。所优化的牛马藤多糖提取工艺稳定可行,提取率高,牛马藤多糖具有一定的抗氧化活性。To clarify the extraction process conditions and antioxidant activity of polysaccharides from Mucuna sempervirens,provide reference for the development and utilization of polysaccharides from Mucuna sempervirens,the polysaccharides of Mucuna sempervirens was determined by Phenol Sulfuric acid method,and the extraction process of polysaccharides from Mucuna sempervirens was optimized through single factor experiments(with extraction temperature,liquid-solid ratio,extraction time,and extraction frequency as reference factors)and orthogonal experiments,the antioxidant activity of polysaccharides from Mucuna sempervirens was determined by DPPH and ABTS methods.The optimized extraction process of polysaccharides from Mucuna sempervirens was stable and feasible,with a high extraction rate,and the polysaccharides from Mucuna sempervirens had certain antioxidant activity.

关 键 词：牛马藤多糖 提取工艺 正交试验法 抗氧化活性 DPPH法 ABTS法 

分 类 号：R917[医药卫生—药物分析学]