曙红探针显色光度法检测氟喹诺酮类兽药残留  

Spectrophotometric Detection of Fluoroquinolones Veterinary Drugs Residues with Eosin Chromogenic Probe

作　　者：郭金科 赵秋伶 韩福 万诚文 GUO Jin-ke;ZHAO Qiu-ling;HAN Fu;WAN Cheng-wen(School of Biomedical and Chemical Engineering,Liaoning Institute of Science and Technology,Liaoning Benxi 117004,China)

机构地区：[1]辽宁科技学院生物医药与化学工程学院,辽宁本溪117004

出　　处：《广州化工》2024年第11期59-62,共4页GuangZhou Chemical Industry

基　　金：国家自然科学基金项目(No.21702086);大学生创新创业训练项目(D202110101443239236)。

摘　　要：曙红和氟喹诺酮类兽药(FQs)作用生成离子对缔合物,溶液由琥珀色变成玫红色,吸收光谱红移。通过测定离子型缔合物在546 nm处的吸光度,建立了FQs残留的检测方法。以恩诺沙星为基准物质建立标准曲线,线性范围为1~20μmol/L,检测限为0.33μmol/L。用裸眼观察结果,检测限为4μmol/L。应用本方法检测实际样本,结果显示:10个猪肝样本里有8个检出FQs残留,残留量在128~655μg/kg之间;10个鸡蛋样本里有6个检出FQs残留,残留量在184~937μg/kg之间(均以恩诺沙星计)。Eosin reacted with fluoroquinolones(FQs)to form ion-pair complexes,the solution changed from amber to rose red,and the absorption spectrum shifted red.A method for the determination of FQs residues was established by measuring the absorbance of ion-pair complexes at 546 nm.The standard curve was established with enrofloxacin as standard substance,and the linear range was 1~20μmol/L,the detection limit was 0.33μmol/L.When observing the results with the naked eye,the detection limit was 4μmol/L.Using this method to detect actual samples,the results showed that FQs residues were detected in 8 of 10 pig liver samples,and the residual amount was 128~655μg/kg,FQs residues were detected in 6 out of 10 egg samples,and the residual amount was 184~937μg/kg(calculated by enrofloxacin).

关 键 词：曙红 氟喹诺酮 兽药残留 分光光度法 

分 类 号：TQ016.1[化学工程] O657.3[理学—分析化学]