地参多糖的制备及其对高糖诱导大鼠肾小球系膜细胞的影响  

Preparation of Lycopus lucidus Polysaccharide and Its Effects on High Glucose-Induced Rat Glomerular Mesangial Cells

作　　者：邱竞瑶 王欣 李春煦 王福生 Qiu Jingyao;Wang Xin;Li Chunxu;Wang Fusheng(College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2024年第8期20-25,共6页Journal of Dali University

基　　金：国家自然科学基金项目(82260679);云南特色植物提取实验室开放研究项目(YKKF2323005)。

摘　　要：目的:探讨地参多糖不同醇沉组分的制备工艺,考察其对高糖诱导大鼠肾小球系膜细胞(HBZY-1)的影响。方法:地参水提液经脱蛋白、大孔树脂柱层析分离,得地参粗多糖,对地参粗多糖进行分级醇沉获得待测组分,以高糖诱导HBZY-1为模型细胞进行体外活性测试。结果:得到的3个不同醇沉组分(LLP-30%、LLP-60%、LLP-90%)在0.25~4.00 mg/mL浓度下,对正常HBZY-1细胞均无毒性;LLP-60%、LLP-90%对高糖诱导HBZY-1细胞的增殖具有显著抑制作用(P<0.01),且能促进细胞超氧化物歧化酶含量上升,丙二醛和活性氧含量降低。结论:地参多糖组分的提取和分离制备工艺切实可行,其中活性组分LLP-90%不仅含量最高,而且抑制高糖诱导HBZY-1细胞的增殖及缓解细胞氧化应激效果最为显著,为地参作为糖尿病肾病功能性食品开发提供基础。Objective:To investigate the preparation process of Lycopus lucidus polysaccharides(LLP)with different ethanol precipitation fractions and evaluate their effects on high glucose-induced rat glomerular mesangial cells(HBZY-1).Methods:The crude LLP was obtained by deproteinization and macroporous resin column chromatography,and the components were obtained by fractional ethanol precipitation.The activity of high glucose-induced HBZY-1 cells was tested in vitro.Results:Three different ethanol precipitation fractions(LLP-30%,LLP-60%,and LLP-90%)showed no toxicity to normal HBZY-1 cells at concentrations ranging from 0.25 to 4.00 mg/mL.LLP-60% and LLP-90%exhibited significant inhibitory effects on the proliferation of high glucose-induced HBZY-1 cells(P<0.01)and promoted the content of superoxide dismutase while reducing the contents of malondialdehyde and reactive oxygen species.Conclusion:The extraction,separation and preparation process of LLP are feasible,and the active component LLP-90% not only has the highest content,but also exhibits the most significant effects on inhibiting the proliferation of high glucose-induced HBZY-1 cells and alleviating cellular oxidative stress,which provided the basis for the development of L.lucidus as a functional food of diabetic nephropathy.

关 键 词：地参多糖 分级醇沉 肾小球系膜细胞 增殖 氧化应激 

分 类 号：R914[医药卫生—药物化学]