多蔗糖酯K326近等基因系的选育及性状分析  

Breeding and trait analysis of near-isogenic lines of K326 for different types of sucrose esters

作　　者：滕环瑜 任书乐 徐寒池 高嘉宁 王召军 崔红[1] TENG Huanyu;REN Shule;XU Hanchi;GAO Jianing;WANG Zhaojun;CUI Hong(College of Tobacco Science,Henan Agricultural University,Zhengzhou 450046,China;Shiyan Branch of Hubei Provincial Tobacco Monopoly Bureau,Shiyan 442000,Hubei,China)

机构地区：[1]河南农业大学烟草学院,郑州市450046 [2]湖北省烟草专卖局(公司)十堰市公司,湖北省十堰市442000

出　　处：《烟草科技》2024年第6期24-32,共9页Tobacco Science & Technology

基　　金：中国烟草总公司科技项目“湘烟7号病毒病抗性及叶面腺毛致香物的聚合定向改良”[110202201010(JY-10)]。

摘　　要：为定向改良K326品种腺毛分泌物中的蔗糖酯组分,以高分泌型烟草(Nicotiana tabacum)种质T.I.1068为供体亲本,烤烟品种K326为轮回亲本,进行多代回交及自交,各个世代利用Ⅲ-Ⅴ型蔗糖酯合成基因(BMVSE)连锁分子标记进行辅助筛选,结合农艺性状观察和叶面化学成分检测分析,选育出多蔗糖酯的K326近等基因系SEK326。SEK326农艺性状与T.I.1068差异显著,与K326基本一致,但茎叶角有所增大,节距显著增大。SEK326叶面腺毛密度显著低于T.I.1068,较K326提高约0.39倍,且腺头饱满、罗丹明染料着色加深。与K326相比,SEK326中西柏烷二萜及Ⅰ-Ⅱ型蔗糖酯分别降低18.15%和33.48%,但新增了Ⅲ-Ⅴ型蔗糖酯,蔗糖酯总量提高约3.12倍,腺毛分泌物总量显著提高。qRT-PCR分析表明,西柏烷二萜合成关键基因NtCBTS及Ⅰ-Ⅱ型蔗糖酯合成关键基因NtASAT1和NtASAT2的表达水平较K326均有所降低。与T.I.1068相比,SEK326腺毛分泌物总量显著降低,而腺毛分泌物组成成分中缺少了冷杉醇和赖百当二醇;分子检测发现,在SEK326与K326中,赖百当二萜合成关键基因NtCPS2具有相同的单碱基突变(G 1542 T),导致了蛋白翻译提前终止。To direct the effort to improve the sucrose ester composition in the secretions of glandular trichomes of tobacco cultivar K326,the high-secreting tobacco(Nicotiana tabacum)germplasm T.I.1068 was used as donor parent,while the flue-cured tobacco cultivar K326 was used as recurrent parent.Multiple generations of backcrossing and self-pollination were performed,with selection in each generation assisted by molecular markers linked to theⅢ-Ⅴtype sucrose ester synthase gene(BMVSE).Combined with observations of agronomic traits and analysis of leaf surface chemical components,a near-isogenic line of K326 with increased sucrose ester types,named SEK326,was developed.The agronomic traits of SEK326 differed significantly from those of T.I.1068,but were basically similar to those of K326,except for increased stem to leaf angle and significantly increased internode length.The trichome density of SEK326 was significantly lower than that of T.I.1068,but about 0.39 times higher than that of K326.In addition,the glandular head of SEK326 was fuller and more deeply stained with rhodamine dye.Compared to K326,the contents of cembranoid diterpenes in SEK326 decreased by 18.15%,and the contents of typeⅠ-Ⅱsucrose esters decreased by 33.48%.However,with the addition of typeⅢ-Ⅴsucrose esters,the total amount of sucrose esters increased by approximately 3.12 times,and there was a significant increase in the content of glandular trichome secretion.qRT-PCR analysis showed that the expression levels of the key gene NtCBTS for cembranoid diterpene synthesis and the key genes NtASAT1 and NtASAT2 for typeⅠ-Ⅱsucrose ester synthesis were all decreased compared to K326.Compared to T.I.1068,the total amount of glandular trichome secretion was significantly decreased in SEK326,and Z-abienol and labdene diol were absent from its composition.Molecular detection revealed that both SEK326 and K326 had the same single nucleotide mutation(G 1542 T)in the key gene NtCPS2 for labdene diterpene synthesis,resulting in premature termination

关 键 词：烟草 腺毛 腺毛分泌物 蔗糖酯 蔗糖酯合成基因(BMVSE) 

分 类 号：S572.03[农业科学—烟草工业]