不同部位血管舒缩反应性与多不饱和脂肪酸的血管活性作用分析  

Vascular Contraction and Relaxation Responsiveness of Different Vascular Beds and Effects of Polyunsaturated Fatty Acids

作　　者：吴逸飞 杨建峰 陈虹[1] 王淼[1] WU Yifei;YANG Jianfeng;CHEN Hong;WANG Miao(State Key Laboratory of Cardiovascular Disease,Fuwai Hospital,Chinese Academy of Medical Science&Peking Union Medical College,Beijing 100037,China)

机构地区：[1]中国医学科学院北京协和医学院国家心血管病中心阜外医院心血管疾病国家重点实验室,北京100037

出　　处：《中国分子心脏病学杂志》2024年第3期6121-6127,共7页Molecular Cardiology of China

基　　金：国家重点研发计划政府间重点专项(2023YFE0118800);国家重点研发计划主动健康和老龄化科技应对重点专项(2020YFC2008003);国家自然科学基金重点国际合作基金项目(82320108002);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-016);中国医学科学院阜外医院高水平医院临床科研业务费(零余额2022-GSP-GG-8)。

摘　　要：目的评价小鼠不同部位血管床的收缩和舒张反应性,比较多不饱和脂肪酸花生四烯酸(AA)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)的血管活性作用。方法分离小鼠股动脉(FA)、主动脉(AO)、肠系膜动脉(MA)、冠状动脉(CA),用微血管张力仪评价其对苯肾上腺素(PE)、U46619的收缩反应性和对乙酰胆碱(ACh)的舒张反应性,拟合剂量-效应曲线,对内皮功能良好的血管进行评价,比较AA、EPA、DHA的作用。结果各血管对U46619的收缩程度比对PE的收缩程度大,PE不收缩CA。以U46619作预收缩剂,比较各血管对ACh的舒张反应,发现MA的舒张程度最大,CA的舒张最不敏感。3种多不饱和脂肪酸诱导CA舒张的程度最小,诱导MA舒张的程度最大、最敏感。与AA和EPA相比,DHA诱导各血管的舒张作用更敏感。结论小鼠不同部位血管床的舒缩反应性有明显差异,各血管均显示对3种多不饱和脂肪酸的舒张反应,其中对DHA的舒张效应最敏感。Objective To evaluate contraction and relaxation responses of different vascular beds of mice,and to compare the vasoactive effects of the polyunsaturated fatty acids-arachidonic acid(AA),eicosapentaenoic acid(EPA),and docosahexaenoic acid(DHA).Methods Femoral artery(FA),aorta(AO),mesenteric artery(MA),and coronary artery(CA)of male and female mice were isolated.Vasocontraction to phenylephrine(PE)and U46619 and vasorelaxation responses to acetylcholine(ACh)were measured by wire myography,with each dose-effect curve fitted,and responses to AA,EPA,or DHA were evaluated and compared in vessels with good endothelial function.Results Each vessel contracted greater to U46619 than to PE,which did not contract CA.Comparing the vasorelaxation responses to ACh among vessels,MA exhibited the greatest vasorelaxation and CA exhibited the least potency.CA exhibited the least vasorelaxation,and MA exhibited the greatest vasorelaxation and the least potency.Compared with AA and EPA,DHA exhibited less potency in each vessel.Conclusion There is significant difference in vasocontraction and vasorelaxation to different vascular beds of mice.Three polyunsaturated fatty acids induce vasorelaxation to all vessels,and DHA exhibitis the greatest potency of vasorelaxation.

关 键 词：多不饱和脂肪酸 血管舒张 花生四烯酸 二十碳五烯酸 二十二碳六烯酸 

分 类 号：R331.32[医药卫生—人体生理学]