添加物对湖北花楸组培增殖苗生长和生根的影响分析  

Effects of additives on the growth and rooting of tissue culture proliferating seedlings of Sorbus hupehensis

作　　者：刘良梦 宋乾丽 黄振[1] 刘欣 陈炙[1] 李佳蔓[1] 陈萍[1] LIU Liangmeng;SONG Qianli;HUANG Zhen;LIU Xin;CHEN Zhi;LI Jiaman;CHEN Ping(Sichuan Key Laboratory of Ecological Restoration and Conservation for Forest and Wetland,Sichuan Academy of Forestry,Chengdu 610081,China)

机构地区：[1]四川省林业科学研究院,森林和湿地生态恢复与保育四川省重点实验室,成都610081

出　　处：《四川林业科技》2024年第4期13-19,共7页Journal of Sichuan Forestry Science and Technology

基　　金：四川省林草科技创新团队项目(2023LCTD0208);四川省基本科研业务费项目(2022JBKY12)。

摘　　要：以湖北花楸5代以上的增殖芽苗为外植体,研究了提高不定芽诱导和生长的方法。结果表明,23种添加物中,琼脂糖和魔芋粉能显著提高芽苗生根率,其中添加魔芋粉的培养基生根效果最好。在含有1 g·L^(-1)魔芋粉的DKW+0.5 mg·L^(-1) NAA的生根培养基中,湖北花楸芽苗生根率由对照的31.67%显著提升至52.86%,当魔芋粉浓度增加至2 g·L^(-1)时,生根率显著提升至80.95%,并可诱导出大量不定根和侧根,平均每株芽苗有5.39条不定根,平均根长21 mm,6.19条侧根,平均侧根长6.7 mm。同时,芽苗茎段伸展,平均苗高可达4.4 cm。Using proliferating seedling of Sorbus hupehensis of more than 5 generations as explants,the method of improving the induction and growth of adventitious buds were studied.The results showed that among the 23 additives,agarose and konjac powder could significantly improve the rooting rate of sprouts,and the medium with added konjac powder had the best rooting effect.In the rooting medium of DKW+0.5 mg·L^(-1) NAA containing 1 g·L^(-1) konjac powder,the rooting rate of Sorbus hupehensis seedlings was significantly increased from 31.67% in the control to 52.86%.When the konjac powder concentration was increased to 2 g·L^(-1),the rooting rate increased significantly to 80.95%,and a large number of adventitious roots and lateral roots could be induced.Each sprout had an average of 5.39 adventitious roots,an average root length of 21 mm,and 6.19 lateral roots,with an average lateral root length of 6.7 mm.Meanwhile,the stem segments of the sprouts stretched,and the average seedling height could reach 4.3 cm.

关 键 词：湖北花楸 魔芋粉 增殖苗 不定根 

分 类 号：S792.25[农业科学—林木遗传育种]