抗生素抗性基因检测方法发展历程及应用现状  

Development history and current applications of methods for detecting antibiotic resistance genes

作　　者：孙凌涛 刘子述 胡宝兰[1,2] SUN Lingtao;LIU Zishu;HU Baolan(College of Environmental and Resource Sciences,Zhejiang University,Hangzhou 310058,Zhejiang,China;Key Laboratory of Environment Remediation and Ecological Health,Ministry of Education,Hangzhou 310058,Zhejiang,China)

机构地区：[1]浙江大学环境与资源学院,浙江杭州310058 [2]污染环境修复与生态健康教育部重点实验室,浙江杭州310058

出　　处：《微生物学报》2024年第8期2591-2609,共19页Acta Microbiologica Sinica

基　　金：国家自然科学基金(22193061,22206166);国家重点研发计划(2020YFC1806903)~。

摘　　要：耐药性的传播已引起全球广泛关注,抗生素抗性基因(antibiotic resistance genes,ARGs)检测技术的开发是研究ARGs在环境-动植物-人群中迁移传播的关键。本文梳理了现有核酸检测技术的发展历程及首次应用于ARGs检测的时间节点,并从检测原理、应用优缺点、开发潜力等方面对各技术进行分类综述。在此基础上提出以等温扩增结合CRISPR/Cas技术为核心的ARGs原位快速检测技术的开发及应用前景展望。本综述旨在回顾各技术发展历程的基础上,为ARGs新检测技术的开发及应用提供参考,为耐药性传播的研究及控制提供技术支撑。The spread of antibiotic resistance has aroused global concern.The development of technologies for detecting antibiotic resistance genes(ARGs)is essential for curbing the migration and spread of ARGs from the environment to plants/animals and human populations.This paper describes the development timeline of existing nucleic acid detection technologies and their first applications to the detection of ARGs and summarizes their detection principles,advantages and disadvantages,and development potential.Furthermore,this paper prospects that isothermal amplification combined with CRISPR/Cas might be the core technology for the development of in-situ rapid detection methods.By reviewing the development history of each technology,this paper aims to give insights into the development and applications of technologies for detecting ARGs and provide technical support for the research and control of antibiotic resistance transmission.

关 键 词：抗生素抗性基因 聚合酶链式反应 基因测序 等温扩增 原位快速检测 

分 类 号：R440[医药卫生—诊断学]