木钙添加量对三角褐指藻高值产物产率的影响  

Simultaneously Enhanced Production of High-Value Products from Phaeodactylum tricornutum by Calcium Lignosulfonate Addition

作　　者：杨泽雄 杨润青 魏东[1] YANG Zexiong;YANG Runqing;WEI Dong(School ofFood Science and Engineering,South China University ofTechnology,Guangzhou 510640,China)

机构地区：[1]华南理工大学食品科学与工程学院,广东广州510640

出　　处：《食品与生物技术学报》2024年第6期128-134,共7页Journal of Food Science and Biotechnology

基　　金：广东省基础与应用基础研究基金重点项目(2019B1515120002)。

摘　　要：在摇瓶中兼养培养三角褐指藻(Phaeodactylum tricornutum)的过程中,作者系统比较了高质量浓度(10~5000 mg/L)和低质量浓度(2.5~50.0 mg/L)木钙对三角褐指藻生物量和3种高值产物产率的影响,旨在确定木钙的最佳剂量。结果表明,木钙的最适添加质量浓度为10 mg/L,此时生物产率为281.32 mg/(L·d);岩藻黄素(fucoxanthin,Fx)、二十碳五烯酸(eicosapentaenoic acid,EPA)和金藻昆布多糖(chrysolaminarin,Chrl)的产率分别为4.41、10.60、57.24 mg/(L·d),比对照组分别提高了9.98%、1.92%、7.27%,其中Fx和Chrl产率显著提高(P<0.05)。作者采用添加木钙的新型兼养培养方式,实现了三角褐指藻中3种高值产物产率的同步提高,为提高三角褐指藻规模化培养效率及应用价值提供了技术支撑。This study systematically compared the effect of high concentration(10~5000 mg/L)and low concentration(2.5~50.0 mg/L)of calcium lignosulfonate on the yield of biomass and three high-value products by using mixotrophic Phaeodactylum tricornutum growth in shake flasks with an aim to determine the optimal dose and effect.The results showed that at the optimal concentration of calcium lignosulfonate(10 mg/L),the biomass yield reached to 281.32 mg/(L·d).Additionally,the yields of fucoxanthin(Fx),eicosapentaenoic acid(EPA)and chrysolaminarin(Chrl)were 4.41,10.60 and 57.24 mg/(L·d),respectively,which were 9.98%,1.92%,and 7.27%higher than that in the control group.Notably,the yields of Fx and Chrl significantly increased(P<0.05).The author developed a novel mixotrophic culture model with the addition of calcium lignosulfonate,which was able to achieve a simultaneous yield enhancement of the three high-value products in mixotrophic Phaeodactylum tricornutum,providing a new technical support for improving the large-scale cultivation efficiency and application value of Phaeodactylum tricornutum.

关 键 词：三角褐指藻 木钙 海洋资源 高值化利用 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]